Studies on bioactivity and secondary metabolites of crude extracts of Bidens pilosa L. (Asteraceae): A medicinal plant used in the Transkei region of South Africa
Journal Title: Pakistan Journal of Pharmaceutical Sciences - Year 2016, Vol 29, Issue 3
Abstract
Whole plant-parts of Bidens pilosa were powdered and extracted in concentrated hexane, acetone, ethanol, methanol and water. The extracts were tested for antimicrobial activity against Escherichia coli (25922), Bacillus subtilis (ATCC 6051), Enterococcus faecalis (51299) Staphylococcus aureus (ATCC 29213) and Pseudomonas aeruginosa (ATCC127853), using standard microbiological techniques. Active crude extracts were macerated in concentrated methanol and tested for secondary metabolites including tannins, saponins, alkaloids, cardiac glycosides, anthraquinones, steroids and flavonoids using standard phytochemical procedures. Hexane and methanol extracts demonstrated similar activity producing 8-17 mm and 11-18 mm inhibition zone-diameter ranges respectively. Further analysis for minimum inhibitory concentrations (MIC50) recorded 1.25-20mg/mL and 2.5-20mg/mL for hexane and methanol extracts respectively. The highest zones of inhibition diameters (22-36mm) and lowest MIC50 values (0.0002-0.0006mg/mL) were recorded for Gentamicin, the positive control. Minimum bactericidal concentration (MBC) ranges were between 10-80mg/mL and 0.001-0.005mg/mL for extracts and control antibiotic respectively. With the exception of anthraquinones, the plant crude extracts tested positive for all secondary metabolites analyzed. These results provide scientific basis for the use of B. pilosa in South African traditional medicine. The antibacterial activity reported herein may be attributed to one or more of the 6 secondary metabolites detected in the plant crude extracts.
Authors and Affiliations
Collise Njume , Bomkazi M Gqaza , Carina Rozani , Nomalungelo I Goduka
Keywords
Related Articles
- EP ID EP138955
- DOI -
- Views 79
- Downloads 0
Comparative research for the dietary pattern of patients with esophageal cancer at different developing stages and the daily intake of vitamin A, E and Beta-carotene
This paper discusses the different stages of normal esophageal’s developing to esophageal cancer, and the difference among dietary patterns of patients with esophageal cancer and acceptable daily intake of vitamin...
Biological activities and chemical composition of the stems and roots of Helichrysum oligocephalum DC grown in Iran
Helichrysum has long been used medicinally, proving to be beneficial in treatment of acne, asthma, bronchitis and circulatory problems, and lymphatic system diseases. The objective of this research was to study the...
Purification and partial characterization of a Fructose-binding lectin from the leaves of Euphorbia helioscopia
A lectin was purified from leaves of Euphorbia helioscopia, by a combination of ion-exchange and gel filtration chromatography. On ion exchange using a DEAE- cellulose column in 0.2 M phosphate buffer, pH 7.2, the...
Evaluation of the interaction of vanadium with glutathione in human blood components
Metallo-elements including Vanadium (V) have strong affinity for sulfhydryl (-SH) groups in biological molecules including Glutathione (GSH) in tissues. Because of this fact it was of interest to further investigate the...
Evaluation of hepatoprotective activity of Colocasia esculenta (L. Schott) leaves on thioacetamide-induced hepatotoxicity in rats
The hepatoprotective effect of orally administered leaf aqueous extract of Colocasia esculenta (CCLE) in thioacetamide-induced liver toxicity in rats was investigated in this study. Adult male Wistar rats (weight r...