Studies on bioactivity and secondary metabolites of crude extracts of Bidens pilosa L. (Asteraceae): A medicinal plant used in the Transkei region of South Africa
Journal Title: Pakistan Journal of Pharmaceutical Sciences - Year 2016, Vol 29, Issue 3
Abstract
Whole plant-parts of Bidens pilosa were powdered and extracted in concentrated hexane, acetone, ethanol, methanol and water. The extracts were tested for antimicrobial activity against Escherichia coli (25922), Bacillus subtilis (ATCC 6051), Enterococcus faecalis (51299) Staphylococcus aureus (ATCC 29213) and Pseudomonas aeruginosa (ATCC127853), using standard microbiological techniques. Active crude extracts were macerated in concentrated methanol and tested for secondary metabolites including tannins, saponins, alkaloids, cardiac glycosides, anthraquinones, steroids and flavonoids using standard phytochemical procedures. Hexane and methanol extracts demonstrated similar activity producing 8-17 mm and 11-18 mm inhibition zone-diameter ranges respectively. Further analysis for minimum inhibitory concentrations (MIC50) recorded 1.25-20mg/mL and 2.5-20mg/mL for hexane and methanol extracts respectively. The highest zones of inhibition diameters (22-36mm) and lowest MIC50 values (0.0002-0.0006mg/mL) were recorded for Gentamicin, the positive control. Minimum bactericidal concentration (MBC) ranges were between 10-80mg/mL and 0.001-0.005mg/mL for extracts and control antibiotic respectively. With the exception of anthraquinones, the plant crude extracts tested positive for all secondary metabolites analyzed. These results provide scientific basis for the use of B. pilosa in South African traditional medicine. The antibacterial activity reported herein may be attributed to one or more of the 6 secondary metabolites detected in the plant crude extracts.
Authors and Affiliations
Collise Njume , Bomkazi M Gqaza , Carina Rozani , Nomalungelo I Goduka
Keywords
Related Articles
- EP ID EP138955
- DOI -
- Views 64
- Downloads 0
Assessment of anti-inflammatory, anti-ulcer and neuro-pharmacological activities of Cyperus rotundus Linn.
This article reports the assessment of anti-inflammatory, antiulcer and neuropharmacological activities of crude extract of Cyperus rotundus. The plant exhibited significant property to act as an anti inflammatory...
Production and antioxidant properties of protein hydrolysate from Rastrelliger kanagurta (Indian mackerel)
Fishery waste and by-products are valuable sources of raw material for recovery of antioxidant and bioactive peptides. Due to the increased demand for protein hydrolysates with antioxidative properties by various sectors...
Evaluation of carrier added and no carrier added 90Y-EDTMP as bone seeking therapeutic radiopharmaceutical
The optimum conditions to label ethylenediaminetetramethylene phosphonate (EDTMP) compound with 90y as a potential candidate for bone metastases therapy were investigated. Yttrium-90 is a pure β-emitter and can be...
Antioxidant activity of bulbs and aerial parts of Crocus caspius, impact of extraction methods
Crocus genus (Iridaceae) is comprises approximately 80 species. In this study in vitro antioxidant activities of extracts from C. caspius bulbs and aerial parts were investigated. Ultrasonically assisted extraction...
Association study between 5-HT2A and NET gene polymorphisms and recurrent major depression disorder in Chinese han population
a functional NET T-182C polymorphism (rs2242446) in the promoter region, a synonymous polymorphisms G1287A in the exon 9(rs5569) and a functional serotonin 2A (5-HT2A) receptor (rs6311) genes in the promoter region...