STUDIES ON ELLAGIC ACID AND 4-HYDROXYISOPHTHALIC ACID ISOLATED FROM SWALLOW ROOT (DECALEPIS HAMILTONII)
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2016, Vol 8, Issue 6
Abstract
Objective: The aim of the study was to elucidate the free radical scavenging activity of purified major phenolic acids from swallow root (Decalepis hamiltonii) extract against 2, 2'-azobis-(2-amidinopropane) dihydrochloride (AAPH) induced oxidation in rat erythrocyte membrane and tissues.Methods: Major phenolic acids, ellagic acid (EA) and 4-hydroxyisophthalic acid (4-HIA) were isolated from aqueous root extract of Decalepis hamiltonii by column chromatography and identified using HPLC, LC-MS, NMR and FT-IR. Free radical scavenging activities of the isolated compounds were estimated. Marker of lipid peroxidation was determined in erythrocyte membrane, liver and brain.Results: The EA showed higher 2, 2-diphenyl-1-picrylhydrazyl (DPPH) and superoxide radical scavenging activities, while 4-HIA exhibited potent hydroxyl radical scavenging activity when compared to standard antioxidants. Malondialdehyde (MDA) levels were increased with AAPH treatment in erythrocytes, liver and brain tissues and the treatment with phenolic acids inhibited the formation of MDA. 4-HIA was found to be more profound in attenuating the lipid peroxidation which may be attributed to its high hydroxyl radical scavenging activity.Conclusions: Our study demonstrated the antioxidant properties of the major phenolic acids isolated from Decalepis hamiltonii and further suggests the therapeutic potential of these compounds in treating the oxidative stress disorders.Keywords: AAPH, Brain, Decalepis hamiltonii, Erythrocytes, Liver, MDA
Authors and Affiliations
Ramachandre Gowda Sowbhagya, Rangaswamy Lakshminarayana, Bhikku Shanbhag Raghuveer, Tekupalli Ravikiran
Keywords
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