Study genetic diversity in maize inbreds and improved hybrid vigor in hybrids produce to determine commercial hybrids in Iraq
Journal Title: JOURNAL OF ADVANCES IN BIOTECHNOLOGY - Year 2015, Vol 5, Issue 3
Abstract
A field experiments was conducted in greenhouse to determinate the genetic diversity among 7 genotypes from maize(4 inbreds and 3hybrids) by using molecular markers with Random Amplified polymorphic DNA(RAPD),that shown high level of polymorphism among genotypes of maize ,where the percentage of polymorphism ranged from(66%) and (83.33%) the highest number of polymorphism band (16) and size fragment ranged between (3800 bp) with the primer ( Bnlg 1185 ) and the lowest 180 with the primer( Bnlg 1464).The genetic distance value ranged between (0.3451) and (0.6534) ,where the lowest genic distance between (k1 and k2),while the highest genetic distance between(k4) and (k3xk4).In this study RAPD markers were shown to be powerful to detect genetic diversity and provided us high polymorphism values within genotypes of maize ,also we can conclude for useful those primers for genetic studies in plant breeding programs for developing synthetic cultivars or improved inbreds of maize.
Authors and Affiliations
Ziyad A. Abed
Keywords
Related Articles
- EP ID EP653875
- DOI 10.24297/jbt.v5i3.1542
- Views 88
- Downloads 0
Secondary multiplication of somatic embryos in banana (Musa spp. AAA) in semisolid medium: effect of the culture vessel type and sterilization method
The objective of this work was to evaluate the influence of the type of culture vessel, and sterilization method on secondary multiplication of somatic embryos of the banana cultivar Grande naine (AAA) in semisolid cul...
Organic Compounds Generated in Bioethanol Production from Agave Bagasse
In bioethanol production through lignocellulosic residues fermentations are generated by-products such as organic compounds (OCs). The organic compounds (OCs) had been well studied in wine and beer industry, but little i...
Mutational Analysis of DNASE I Gene in Diabetic Patients
The main aim is to analyze the mutations of DNASE I gene in diabetic patients.A total of 120 diabetes patients and 120 controls were sampled. The total number of male diabetic patients included in the study was 79 (66%)...
Evaluation of protocorm formation in meristematic regions of roots and leaves of Miltonia spectabilis moreliana (Orchidaceae).
The aim of this study was to evaluate the effect of 2.4-D and BAP on protocorm formation in meristematic regions of root and leaf of Miltonia spectabilis moreliana, and to test the effect of sucrose concentrations and pH...
Effect of Plastic Synthetic Aggregate in the Production of Lightweight Concrete
In this research work, the effect of plastic synthetic aggregate in the production of lightweight concrete was studied. The plastic synthetic aggregate was used to replace 0-40% of coarse aggregates. A mix proportion of...