STUDY OF ANTIADENOVIRALACTIVITY OF NEW FLUORINECONTAINING HETEROCYCLIC COMPOUNDS
Journal Title: Мікробіологія і біотехнологія - Year 2014, Vol 0, Issue 1
Abstract
Purpose: To study the cytotoxic action and antiviral activity of new fluorinated nucleoside compounds on human adenovirus model and the role of individual molecular fragments in their activity. Methods. Cytotoxic activity of the compounds was determined by the MTT method. Antiviral activity of substances was studied using cyto-morphological method with acridine orange dye. The virus-specific intranuclear inclusions were determined. Results. In present study the cytotoxicity of 2N-substituted-4-tosyl-5-polyfluoroalkyl-1,2,3-triazoles for Hep-2 and Hela cell lines were studied. The dependence of cytotoxicity on the compounds in the structure of substituents in the aromatic rings of the molecules was defined. Combination of residues of 3-hlorotetrahydropyran, 3-chloro-dihydrofuran tetrahydrofuran in a glycosidic moiety with trifluoromethyl or perfluoropropyl in a triazole ring were more toxic. Antiviral activity of 3 compounds (G7, G8 and G10) against HAdV5 in vitro was revealed. The effective antiviral concentrations (EC50) for them were 16, 70 and 186 mg/ml, and the selectivity indexes were equal to 91, 8 and 4, respectively. Conclusions. High rates of antiviral activity and low cytotoxicity of G8 compound make it perspective for development antiviral agents.
Authors and Affiliations
L. O. Biliavsk, O. Yu. Povnitsia, Yu. G. Shermolovich, G. P. Gudz, N. V. Nesterova
Keywords
Related Articles
- EP ID EP395235
- DOI 10.18524/2307-4663.2014.1(25).48195
- Views 92
- Downloads 0
EFFECT OF LOW-INTENSITY LASER IRRADIATION ON THE CULTIVATED MACROMYCETES SEED CULTURE GROWTH ACTIVITY
Aim. The study of the effect of low-intensity laser radiation on the growth processes of spores and mycelia of cultured macromycetes. Methods. The research objects were pure cultures of basidiomycetes Flammulina velutipe...
BIOFILM FORMATION BY PSEUDOMONAS AERUGINOSA STRAINS WITH DIFFERENT LEVELS OF CYCLIC DI-GUANOSINE MONOPHOSPHATE BIOSYNTHESIS
Aim: Swarming motility and biofilm formation abilities determination in P. aeruginosa PA01 pJN2133, with low c-di-GMP level. Materials and methods. P. aeruginosa PA01 and P. aeruginosa PA01 pJN 2133 were used as a test-o...
STUDY OF ANTIADENOVIRALACTIVITY OF NEW FLUORINECONTAINING HETEROCYCLIC COMPOUNDS
Purpose: To study the cytotoxic action and antiviral activity of new fluorinated nucleoside compounds on human adenovirus model and the role of individual molecular fragments in their activity. Methods. Cytotoxic activit...
EFFECT OF ANTIBIOTICOTHERAPY ON THE TOTAL AMOUNT OF OXALATE-DEGREADING BACTERIA IN THE RATS INTESTINAL TRACT
Aim. To determine the quantity of oxalate-degrading bacteria (ODB) in fecal biopsy and mucosa-associated microbiota of the colon and small intestine of rats. Methods. Study was conducted on male Wistar rats (weight 170–2...
MICROBIAL DIVERSITY OF COASTAL WATERS OF ODESA BAY OF THE BLACK SEA
The aim of the study was to determine the biodiversity of the prokaryotic microbiota of the coastal water of Odesa Bay of the Black Sea with the help of metagenomics analysis. Methods. Sample of sea water was taken in th...