Study of biofilm producing capacity of pathogens isolated from different catheters
Journal Title: Medpulse International Journal of Microbiology - Year 2017, Vol 4, Issue 1
Abstract
Background: Persistent infection due to biofilm formation is certainly a new and additional burden to clinicians who treat infections. The purpose of this study is to determine the biofilm producing capacity of pathogens isolated from different catheters. Material and Methods: A total of 109 catheter samples from 100 adult patients of both sexes admitted to the Intensive Care Units (ICUs) who have been catheterized for more than 48 hrs showing clinical signs of sepsis. Maki’s semi-quantitative method was used to culture thecatheters and biofilm detection was done by culture plate assay. Results: Out of the 109 catheters studied and cultured, a total of 26 isolates were obtained. Coagulase negative staphylococci and Escherichia coli formed the most commonly isolated pathogens at 5 (19.2%) each. Among the 26 isolates cultured, 22 of the organisms produced biofilms as evidenced by the tissue culture plate assay. Conclusion: Biofilm formationpose a public health problem for the persons who require indwelling medical devices. Its detection should be mandatory in a laboratory set up. Tisue culture plate method is an accurate and reproducible screening method for biofilm production.
Authors and Affiliations
Harsha Vardhana S, P R Sreenivasa Babu, M R Sandhya Belawadi
Keywords
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- EP ID EP487286
- DOI 10.26611/1008414
- Views 74
- Downloads 0
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