STUDY OF CARBAPENEM RESISTANCE IN NON-FERMENTING GRAM NEGATIVE ISOLATES
Journal Title: Journal of Evidence Based Medicine and Healthcare - Year 2015, Vol 2, Issue 34
Abstract
The introduction of Carbapenems (Meropenem, Imipenem) into clinical practice represented a major advance in the treatment of serious bacterial infections caused by beta-lactam resistant bacteria. However, carbapenem resistance has been frequently observed in non-fermenting bacilli like Pseudomonas aeruginosaand Acinetobacter species, probably due to lack of drug penetration and/or carbapenem-hydrolyzing β-lactamases. OBJECTIVES: The objectives of the present study are to isolate nonfermenters from various clinical samples, determine their antibiogram and to detect any carbapenem resistance by the application of routine phenotypic methods. MATERIALS AND METHODS: A total of 132 samples were collected from 132 patients samples includes pus, urine, sputum, blood, endotrcheal tubes, intravenous cannula isolated non-fermenters from various clinical samples and identifying the organism their antimicrobial susceptibility testing was performed by Kirby-Bauer disc diffusion method. Metallo-β-lactamase (MBL) detection was done by the Imipenem-EDTA Combined Disc Method and the Imipenem-EDTA Double Disc Synergy Method. RESULTS: Among 132 isolates, Pseudomonas aeruginosa constituted 115 of the total isolates, Imipenem resistance in the present study in Ps. aeruginosa was 41.73%. Among the 57 isolates resistant to imipenem, 50(87.71%) showed a ≥7mm zone enhancement by Imipenem-EDTA Combined Disc Method, 35 (61.4%) showed zone enhancement by the Imipenem-EDTA Double Disc Synergy Method. CONCLUSION: Among the two tests used in the present study, the Imipenem-EDTA Combined Disc Method was found to be more sensitive (p<0.05). MBL E test can be used to increase the sensitivity of detection
Authors and Affiliations
Syed Haroon Hussain, Sukesh Kumar B. Y. , Kondal Rao
Keywords
Related Articles
- EP ID EP231465
- DOI 10.18410/jebmh/2015/713
- Views 86
- Downloads 0
EASE OF ACCESS TO THE SUBARACHNOID SPACE AND ITS ASSOCIATION WITH SPINOUS PROCESS DIMENSIONS, IN PATIENTS UNDERGOING SPINAL ANAESTHESIA
BACKGROUND Spinal anaesthesia is a procedure commonly performed by the anaesthesiologist. Various reasons can lead to a difficulty in performing a lumbar puncture, leading to multiple attempts, thereby increasing chances...
EFFECTIVENESS OF SEPRAFILM AS ADHESION BARRIER FOLLOWING ABDOMINOPELVIC SURGERY- AN EXPERIMENTAL STUDY
BACKGROUND Postoperative adhesions form one of the most common complication following abdominopelvic surgeries. The consequence of adhesion leads to lifelong morbidity and form major burden in surgeons operating for the...
COMPARATIVE STUDY OF 0.125% BUPIVACAINE HYDROCHLORIDE VERSUS 0.125% BUPIVACAINE HYDROCHLORIDE WITH 25 µgms FENTANYL CITRATE FOR EPIDURAL ANALGESIA IN LABOUR
BACKGROUND Labour pains are said to be very intense for many women. They may adversely affect both mother and foetus. A variety of techniques are available to offer labour analgesia. Of all techniques, continuous epidura...
CLINICAL EVALUATION OF ORAL KETAMINE AND ORAL MIDAZOLAM FOR PREMEDICATION IN PAEDIATRIC SURGICAL OUTPATIENTS
BACKGROUND Forty children of ASA grade I, aged 1 to 8 years, both males and females undergoing elective surgery under general anaesthesia and regional anaesthesia for procedures such as herniotomy and circumcision were i...
A STUDY OF PREGNANCY OUTCOMES AND PLACENTAL MORPHOLOGY ASSOCIATED WITH PREGNANCY-INDUCED HYPERTENSION
BACKGROUND Pregnancy-Induced Hypertension (PIH) contributes significantly to maternal and perinatal morbidity and mortality. Women with PIH are at a greater risk of abruptio placentae, cerebrovascular events, organ failu...