Study of Phenytoin Effect on the genes involved in glucose and lipid metabolism expression in liver: A mouse model study
Journal Title: Journal of Current Biomedical Reports - Year 2021, Vol 2, Issue 2
Abstract
Phenytoin as an anti-seizure medication, is useful for the prevention of tonic-clonic seizures and focal seizures. In this study we focused on the probable effects of Phenytoin drug on gene expression profile of liver related to lipid metabolism balance in mouse as a model. In this study, a group including 7 male mice of BALB/c were treated with phenytoin 3–5 mg/kg/day orally and a group including 7 male mice of BALB/c were took standard food. Liver tissue samples were isolated. Total RNA was extracted and cDNA was synthesized. Expression of Akt1, Leptin, Adipoq and GLUT4 genes was measured using Real-time RT-PCR method. Results showed an increase about 15 and 3 fold changes in Akt1 and Adipoq gene expression respectively in treatment group compare to control mice. Also, we detected decreasing in Leptin and GLUT4 genes expression in the mice treated with phenytoin drug. Several studies indicated that phenytoin can promote hyperglycemia in human and animal. We proposed here that this effects may resulted from an interference between the phenytoin drug and gene expression profile in liver. Decreasing of leptin level here may be a result of glucose level elevation in blood that can induce a satiety situation result in decrease of leptin production. It may that Akt1 gene expression is increased to compensate the low level of GLUT4 protein. We concluded that phenytoin is a relatively high-risk antiepileptic drug for obesity and metabolic syndrome, but more studies are needed.
Authors and Affiliations
Fatemeh Samimi, Ali Mohammad Ahadi, Nafiseh Boroomand, Farzaneh Mohammadzadeh Rostami
Case reports in the use of vitamin C based regimen in prophylaxis and management of COVID-19 among Nigerians
This case study was based on the novel opinion that daily intake of vitamin C can serve as a prophylactic and curative intervention for the coronavirus disease of 2019 (COVID-19) via homeostatic and epigenetic mechanisms...
Molecular investigation of extended-spectrum β-lactamases (ESBLs) genes in the Salmonella isolates obtained from children with acute diarrhea
Salmonellosis is an important public health concern among children in worldwide. Extended-spectrum β-lactams (ESBLs) cause resistance to clinically important beta-lactams which are generally used to treat invasive Salmon...
The sound of getting rid of coronavirus by RNA interference technology: RNAi against COVID-19
No Abstract.
Current applications of the microbiome engineering and its future: A brief review
In the human body there are many microorganisms with a variable genetic content. These microorganisms play an important role in the metabolism, homeostasis, immune system and generally human health. Over the millions of...
The role of immunotherapy in eliminating high-risk treatments for pediatric cancer
Chemotherapy, radiation, and surgery are often ineffective in treating refractory or chronic tumors in children. Recent advances in cancer immunotherapy have also improved the outcome of many human tumors, resulting in s...