Study of Xylanolytic Microorganisms by using Xylan Extracted from Natural Waste
Journal Title: International Journal for Research in Applied Science and Engineering Technology (IJRASET) - Year 2017, Vol 5, Issue 5
Abstract
The novel approach used in current research is use of extracted xylan from natural wastes like wheat bran and Pistia sp for screening of xylanolytic microorganisms. Xylan recovered from extraction of wheat bran was 22.2% and Pistia sp was 23.7%.A total of 36 isolates were obtained from different soil samples collected from Kolhapur District. Out of these, B2 isolate showed 27mm diameter of zone of clearance on medium containing pure xylan, while on medium containing Pistia sp and wheat bran showed 27mm and 28mm respectively. This B2 isolate was identified as Massilia timonae after 16s rRNA sequencing and the new strain was named as Massilia timonae B2YR.Its 16s rRNA nucleotide sequence was deposited in GenBank under accession number KY942185. Along with xylanases the organism also produces cellulase and amylase enzyme.
Authors and Affiliations
R. J. Thanekar, Y. C. Attar
Keywords
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