Study on conditions for gama buryric acid (GABA) production in Lactobacillus fermentum 101 isolated from human.
Journal Title: International Journal of Medical Science and Innovative Research (IJMSIR) - Year 2018, Vol 3, Issue 12
Abstract
Lactic acid bacteria play a vital role in biosynthesis of γ -aminobutyric acid (GABA) in the presence of glutamic acid - major substrate for the process. In the present study, Lactobacillus fermentum (L. fermentum) isolated in various sources in Vietnam that were screened for bacteria strains with high-efficiency in GABA formation. In the study, L. fermentum was cultured in MRS broth containing 25 mg/mL MSG, at pH of 6.5 and incubated at the optimal time (37ºC, for 24, 48, 72 h). After extraction and thin layer chromatography (TLC) and high performance liquid chromatography (HPLC) analysis, L. fermentum 101 showed the GABA yield about 1.34 mg/g dried supernatant, suggesting that L. fermentum 101 was found to be a promising GABA producer for food and pharmaceutical field).
Authors and Affiliations
Nguyen Hoang Khue Tu
Keywords
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