Study on continuous monitoring automated culture systems for rapid isolation of microorganisms
Journal Title: Scholars Journal of Applied Medical Sciences - Year 2017, Vol 5, Issue 6
Abstract
Abstract: Rapid isolation and reliable diagnosis of blood borne infections and characterization of bacterial microorganisms is one of the important tasks for clinical microbiologists. The present study was undertaken to evaluate the Time to Detection of various micro organisms with BACTEC 9120. This study was done on Blood Culture samples obtained from the outpatients as well as admitted patients at IndraprastNadia Sufdar Ali , Lecturer , Department of MLT,College of Applied Medical sciences, Jazan University, Jizan, KSA.ha Apollo Hospital, New Delhi. The BACTEC fluorescent series instruments, BACTEC 9120, are designed for the rapid detection of bacteria and fungi in clinical cultures of blood. Samples are drawn from patient and injected directly into BACTEC culture vials. Positive cultures are immediately flagged by an indicator light on the front of the instrument and displayed on the monitor. When positive vials are identified, the lab technologist pulls them from the instrument for confirmation of results, and for isolation and identification of the organism. Among this 97 blood culture yielded gram positive cocci, 87 yielded gram negative bacilli, 23 were positive for fungi, 36 showed mixed growth and 10 blood culture yielded Gram positive bacilli. All the positive samples from BACTEC 9120 were monitored for positivity and the positive samples were then identified by two methods Vitek-2 and direct methods. In the study it was observed that the maximum percentage of microorganisms isolated within the time period was Gram Positive cocci (37%), followed by Gram Negative Bacilli (33%) and the least isolated was Fungi (9%). From the Time to Detection (TTD) charted out, Fungi were seen taking maximum time to detection (32.4 hrs). This was followed by Gram Positive Cocci (19.3 hrs) and Glucose Non Fermenters (18.4 hrs). The least TTD was shown by Enterobacteriaceae (15.3 hrs). BACTEC 9120 automated Continuous monitoring culture systems are very efficient and important tool in management of critically ill patients and have a great edge over conventional detection in cutting crucial time for detection. Automated Culture, identification and Susceptibility System being versatile system forms inseparable part of management of critically ill patients. Keywords:continuous monitoring automated culture systems, microorganisms
Authors and Affiliations
Nadia Sufdar Ali, Khan Tabassum Tanveer, Muzaffer Ali Khan, Raman Sardana
Keywords
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