STUDY ON PREVALENCE OF METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS; COMPARISON OF FOUR DIAGNOSTIC METHODS FOR DETECTING MRSA– FROM A TERTIARY CARE HOSPITAL, MADURAI
Journal Title: Journal of Evolution of Medical and Dental Sciences - Year 2018, Vol 7, Issue 7
Abstract
BACKGROUND Prevalence of Methicillin-Resistant Staphylococcus aureus (MRSA) in India ranges from 30 to 70% which leads to high mortality, increased economic burden and high treatment failure when compared to MSSA. Rapid and accurate detection of MRSA is essential to take measures for infection control and also to prevent unnecessary use of antibiotics. MATERIALS AND METHODS Prospective observational study was carried out in our tertiary care hospital over a period of 6 months (May-October 2017). All Staphylococcus grown among 240 various clinical samples received were tested for MRSA by four phenotypic methods - cefoxitin and oxacillin disc diffusion test and MIC oxacillin E test and oxacillin resistant screen agar test (ORSA) with mecA-PCR as gold standard. RESULTS A total of 103 out of 200 (51.5%) bacterial isolates were Staphylococcus aureus. Pus from diabetic wound yielded high number of growth (42%) followed by blood (36%) which is alarming note. 55.4% were identified as MRSAs by PCR; most common presenting group was >50 years (36.8%) followed by infants (28%). Cefoxitin disc method has the highest sensitivity and specificity of 98.2% & 100% followed by E test (94.2% & 95.8%) and ORSA (87.5% & 94.2%). Oxacillin disc diffusion method had the least 79.5% & 92.9%. Most MRSA isolates were multi drug resistant. CONCLUSION Cefoxitin disc diffusion when combined with Oxacillin agar or E test will be helpful to detect all MRSA strains including hyper production of betalactamases. As these phenotypic assays are simple and relatively cheap, this can be used as an alternative to PCR in resource constraint settings.
Authors and Affiliations
Rajendran Thiruvannamalai, Jhansi Charles, Raja Sundaramurthy, Ramesh Arunagiri, Vithiya Ganesan, Geni Veerathevar German Soundaram, Brindha Vetrinallathambi
Keywords
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- EP ID EP399086
- DOI 10.14260/jemds/2018/197
- Views 58
- Downloads 0
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