Study the Effects of Helium Neon (He-Ne) Laser Irradiation on Normal Human Blood in Vitro Using FTIR Techniques
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2018, Vol 13, Issue 4
Abstract
Interaction of lasers with biomaterials such as blood, skin, and tissues is an important area of research. Laser tissue interactions can be understood by using spectroscopic and advanced microscopic techniques such as scanning electron microscope (SEM). In the present, work blood samples were collected from normal human subjects under standard laboratory conditions. Blood samples were irradiated by He-Ne laser (Wavelength λ = 632.8 nm, Power P = 3mW). The FTIR spectra for non-irradiated normal blood samples are compared with the FTIR spectra of irradiated blood samples. Significant changes are observed between the various bonds from the FTIR transmission spectra between C=O (Amide I), C-O (Anhydrides), N=O (Nitro), C-N (Amines) and C-H (Alkenes) etc. The significant results are obtained when He-Ne laser irradiation is incident on whole blood for 30 and 40 minutes and the transmittance decreases due to denaturation of proteins. The present work will provide more inside into laser irradiation - blood interactions and form a basic for therapeutic uses.
Authors and Affiliations
V. H. Ghadage, B. N. Zaware
Keywords
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- EP ID EP395455
- DOI 10.9790/3008-1304010105
- Views 75
- Downloads 0
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