Subtractive library obtained from a mutant of variety B 4362 with resistance to sugar cane rust
Journal Title: Biotecnología Vegetal - Year 2002, Vol 2, Issue 3
Abstract
The hypersensitive response is one of the most powerful mechanisms for which the plants resist pathogen attack. Mutations carried out previously on the variety B4362, of sugarcane, originated five mutants that express this mechanism towards the attack of rust (Puccinia melanocephala Syd.). By means of a subtractive hybridization among the cDNA obtained starting from the resistant clone inoculated with rust and a pool of cDNA of the susceptible variety (B4362) inoculated and of the resistant clone not inoculated, it was possible to reduce the number of genes expressed during the infection with the fungus. A subtractive library was carried out where we hope that most of the genes are involved in the hypersensitive response that present these mutants towards the infection of the pathogen.
Authors and Affiliations
María I. Oloriz, Luis Rojas, Víctor Gil, Elio Jiménez
Keywords
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