Sweet Potato (Ipomoea batatas L.): Suspension Culture for cell Aggregation
Journal Title: Journal of Plant Science and Research - Year 2017, Vol 4, Issue 2
Abstract
Cell aggregation in suspension culture of sweet potato (Ipomoea batatas L.) was established from petiole-derived callus. The highest rate (55%) of petiole induced callus on agar-gelled Murashige and Skoog (MS) medium containing 10 µM 2,4-Dichlorophenoxyacetic acid (2,4-D) within 30 days of culture. These calli were used for preparation of suspension culture in liquid medium containing same concentration of 2,4-D that was used for callus induction. A growth curve with lag, exponential and stationary phages of cell growth was obtained when total amount of cell was plotted against the culture periods. Cells underwent divisions, resulting in free cells multiplication and cell aggregates formation after further 30 days of culture. The initial amount of cell during suspension culture showed effect in the cell proliferation, cell size and cell aggregate development. The additional amount of cell and cell aggregates formation were the highest when 5 ml Sedimented Cell Volume (SCV) of cells was cultured. The cell proliferation reduced and high number of elliptical cell was found in suspension when culture maintained for longer period without transferring to fresh medium.
Authors and Affiliations
Mahabubur Rahman
Keywords
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