Synergism effect of rosemary essential oil and some antibiotic against Escherichia coli isolated from clinical samples
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2017, Vol 12, Issue 2
Abstract
Urinary tract infections (UTI) are among the most common infections in humans worldwide, E. coli strains in particular are responsible for 80% of the UTI cases. Therefore, the study aimed to investigate the antimicrobial activity of Rosemary essential oil (EO) and three most antibiotics used for UTI treatment as well as determine the best combinations between the EO and these antibiotics against E. coli isolated from urine samples. The ability to form biofilm formation was performed and antimicrobial activity for antibiotics and rosemary EO were investigated by minimal inhibitory concentration (MIC) in broth microdilution method. The results showed that all isolates had the ability to produce biofilm (100%). The MIC value of gentamicin, trimethoprim/sulfamethoxazole, ciprofloxacin and Rosemary EO against E.coli were 256, 2000, 500 and 104 µg/ml, respectively for strong biofilm producer isolates while the MIC values were 512, 2000, 1000 and 104 µg/ml, respectively for moderate biofilm producer isolates.Combination of gentamicin and ciprofloxacin with EO were reduced the MIC of antibiotics when used alone in both strong and moderate biofilm producer isolates. In conclusion, the combination of gentamicin and ciprofloxacin with rosemary EO were increased the antimicrobial activity of these antibiotics (synergetic effect) against E. coli isolated from urine.
Authors and Affiliations
Ghusoon A. Abdulhasan
Keywords
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- EP ID EP388691
- DOI 10.9790/3008-1202033942
- Views 57
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