Taq1 Polymorphism (rs731236) of Vitamin D Receptor Gene in Children with Acute Lymphoblastic Leukemia
Journal Title: Iranian Journal of Blood and Cancer - Year 2017, Vol 9, Issue 4
Abstract
Background: Acute lymphoblastic leukemia (ALL) is the most common childhood cancer. Several studies have shown that ALL occurs as a result of genetic abnormalities. 1, 25-Dihydroxyvitamin D3 as a secosteroid hormone plays an important role in different metabolic pathways. The normal function of vitamin D occurs via binding to a ligand-activated transcription factor i.e. vitamin D receptor (VDR). Therefore genetic variation in VDR may lead to various disorders, including cancer. We aimed to investigate rs731236 polymorphism of VDR gene in children with ALL. Methods: Genomic DNA was extracted from 50 children under 15 years of age with ALL and 50 age-matched healthy children’s whole blood and genetic variation of each participant was detected using polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) with taqI restriction enzyme. Statistical analysis was performed using SPSS v.22 following χ2 test. Results: The VDR gene polymorphisms were genotyped in a total of 50 individuals with ALL, comparing 50 normal children with a mean age of 5.2±3.4 years. No deviation was observed from Hardy-Weinberg equilibrium (HWE) in the genotypic distribution of the rs731236 (χ2=0.25, P>0.05). Genotype frequency of TT (Dominant Homozygote), Tt (Heterozygote), and tt (Recessive Homozygote), was 25, 15 and 10 in the case group and 21, 27 and 2 in the control group, respectively which showed a significant difference between two groups (P=0.011). tt genotype showed a strong protective effect against ALL over Tt (OR=9, CI 95%: 1.74-46.59). Conclusion: We have determined the frequency of Taq1 (rs731236) polymorphism in the VDR gene in children with ALL in Zanjan population. We concluded that genotype variation in VDR gene may have an effect on incidence of ALL.
Authors and Affiliations
Atiyeh Ale Ahmad, Ehsan Saburi, Toktam Rajaei, Yousef Mortazavi
Keywords
Related Articles
- EP ID EP281306
- DOI -
- Views 122
- Downloads 0
Density of Platelet GPIIb-IIIa and Bleeding Severity in Iranian Patients with Glanzmann’s Thrombasthenia
Background: Glanzmann’s Thrombasthenia (GT) is a rare autosomal, recessive, bleeding syndrome. The main aim of this study was to investigate the relationship between symptoms, bleeding severity, and gender and subtypes o...
Treatment of a Child with Refractory Acute Myeloid Leukemia with Humanized Anti-CD33 Monoclonal Antibody: A Case Report and Review of Drug Development
Background: The induction chemotherapy regimen for acute myeloid leukemia has evolved as once induction is completed patients progress through the consolidation phase and achieve remission in 76% of cases. For patients w...
Investigation of Leptin, Leukemia Inhibitory Factor (LIF), and IL-6 Serum Levels in Myeloid Leukemia
Background: Leptin has been implicated in the differentiation and proliferation of hematopoietic cells. Leukemia inhibitory factor (LIF) may play an important role, along with Interleukin-6 (IL-6) and granulocyte colony...
Prenatal Diagnosis and Frequency Determination of alpha and beta Thalassemia, S, D, C, and H Hemoglobinopathies Globin Mutational Genes Aanalysis among Voluntary Couples from Ahvaz
Background: The aim of this perspective study was to assess the frequency of hemoglobinopathy mutational genes among voluntary hemoglobinopathy carrier couples-to-be referred to thalassemia center, Shafa hospital, affili...
Quantitative Immunophemotyping of Platelet Surface Glycoproteins among Iranian Patients with Bernard-Soulier Syndrome
Background: Bernard-Soulier syndrome is a rare inherited bleeding disease caused by quantitative or qualitative defect of GPIb/IX/V, a platelet complex that binds the Von Willebrand factor. The expression of GPIb-IX-V co...