The assessment of selected molecules belonging to B7 family on the mature dendritic cells in laryngeal cancer patients
Journal Title: Otolaryngologia Polska - Year 2012, Vol 66, Issue 6
Abstract
B7-I-I1, B7-I-I2 and B71-14 molecules play various roles in the adaptive immune system and are broadly expressed on many cells, especially those localized in cancer tissue. The aim of the study was to assess the surface expression of B7-H1, B74-12 and B71-14 molecules on the mature dendritic cells (DC) generated from peripheral blood monocytes of laryngeal cancer patients and healthy donors. Material and methods: Forty-four male patients treated surgically for squamous cell carcinoma of the larynx were included in the study. Peripheral blood from twelve healthy male donors was used as a control. Mononuclear cells were separated from all individuals by density gradient centrifugation, incubated with anti-CD14 microbeads, and passed through MACS separation columns. The CD14 positive cell popu-lation was used to prepare monocyte derived DC. Laryngeal cancer tissue was obtained from patients during surgical treatment and homogenized to prepare tumor cell lysates for further stimulation. We evaluated with the use of flow cytometry method the percentage of cells with an expression and mean fluorescent intensity (MFI) of surface markers, such as: CD83, B7-H1, B71-12, B71-14. Results: Our study revealed that the percentage of mature dendritic cells, stimulated with autologous tumor cell lysates, with the expression of B7-H1 molecule in patients was lower than in healthy donors (61.81 + 25.58% vs 93.02 + 4.63%, p = 0.007). In laryngeal cancer patients, the percentage of CD83+/B7-I-12+ cells was higher than in healthy individuals (18.32 + 10.74% vs 2.89 + 0.43%, p = 0.019). Conclusions: There is a relation between the presence of laryngeal cancer and the expression of B7 family molecules.
Authors and Affiliations
Janusz Klatka, Ewelina Grywalska, Magdalena Wasiak, Paulina Wdowiak, Adrian Andrzejczak, Eliza Trzaskowska, Jacek Roliński
Keywords
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