Тhe change of flowering genes expression in Arabidopsis thaliana is a response to UV-C irradiation in different light conditions and temperature cultivation conditions
Journal Title: Naukovyi visnyk Chernivetskoho universytetu. Biolohiia (Biolohichni systemy) - Year 2018, Vol 10, Issue 1
Abstract
The main goal of this study is researching how grown conditions act on flowering time and flowering genes expression under stress factors acting. Arabidopsis thaliana plants Col0 ecotype were cultivated in red, violet and orange light at 21оС and 24оС in light and dark mode at a ratio of 18/6. The phases of development were fixed during the growth of plants. In the 2.3 phase, the experimental plant groups were irradiated with UV-C for 10 minutes. In phase 5.1. the relative expression of Apetala 1, Gigantia, Leafy, Flowering locus T, Flowering locus C, Constants, RAD51 and PCNA2 genes were determined in real time PCR using. Gene reference actin Act was used. Phenological data showed that plants which grown in violet and orange and at 21℃ have 6 days developmental stage delayed compared with white light at 24℃; plants grown in red and violet spectra at 24оС have 3 days delayed. An analysis of relative gene expression showed that the RAD51 down regulated in response to UV irradiation at a factor of 0.379 (P (H1) = 0.000, 95% C.I. 0.192 - 0.764) in plants growth in the red light spectrum at 24оС. Expression of the circadian rhythm gene Co down regulated with a coefficient of 0.021 (P (H1) = 0.000, 95% C.I. 0.011 - 0.041), Gi down regulated with a coefficient of 0.291 (P (H1) = 0.000, 95% C.I. 0.144 - 0.595), the expression of AP1 increases with a coefficient of 2.557 (P (H1) = 0.000, 95% C.I. 1.194 - 6.399). The expression of transcription factor AP1 down regulated of a coefficient of 0.023 (P (H1) = 0.000, 95% C.I. 0.007-0.078) and 0.306 (P (H1) = 0.000, 95% C.I. 0.116-0.818), respectively in plants grown in violet and orange light at a temperature of 21оС. The expression of LFY also decreases with the coefficient 0.177 (P (H1) = 0.000, 95% C.I. 0.069-0.455) and 0.33 (P (H1) = 0.000, 95 % C.I. 0,172 - 0,633). For a group of plants cultivated in violet light at 24оС, no statistically significant changes in expression were detected among the genes studied.
Authors and Affiliations
M. V. Kryvokhyzha, N. M. RASHYDOV
Keywords
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- EP ID EP498705
- DOI 10.31861/biosystems2018.01.008
- Views 89
- Downloads 0
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