The Characterization of Amylolytic Enzyme Present in Fermented Sweet Sap of Palmyrah
Journal Title: Journal of Applied Biology & Biotechnology - Year 2016, Vol 4, Issue 3
Abstract
Amylase enzyme obtained from 12h of naturally fermented sweet sap of palmyrah. The amylase showed zero order kinetics for 10min. The optimum temperature for the activities of enzyme as 65oC and pH was 7.0. When the enzyme was pre-incubated at 65oC and at pH 7.0, it lost 62% of its initial activity at 60min. This enzyme showed activity with starch, lactose, maltose, pectin and sucrose. Amylase activity was strongly inhibited by 2mM of Hg2+ but 2 mM of Ca2+, Ba2+, Mg2+, Mn2+, Na+, Zn2+, Mn2+ and Cu2+ stimulated the enzyme activity at 65 oC and at pH 7.0. The enzyme in the presence of 2mM Mn2+, Cu2+ and Na+ separately, retained 79, 86 and 68 % of its initial activity respectively at 60 min, 65oC and pH 7.0.
Authors and Affiliations
Keywords
Related Articles
- EP ID EP456311
- DOI 10.7324/JABB.2016.40304
- Views 80
- Downloads 0
Transcriptional expression of three putative pathogenesis-related proteins in leaves of rubber tree (Hevea brasiliensis) inoculated with Neofusicoccum ribis
Pathogenesis related-proteins (PR-proteins) and enzymes are important tools for understanding the molecular markers of plant response to external factors. In this study, semi-quantitative reverse transcription polymerase...
Comprehensive analysis of damage-associated single-nucleotide polymorphisms of sex hormone-binding globulin gene
Sex hormone-binding globulin (SHBG) controls the bioavailability of androgens, and its association has been found in a number of disorders such as hyperandrogenism, obesity, diabetes, and cancer. Many human singlenucleot...
Progress in understanding the regulation and expression of genes during plant somatic embryogenesis: A review
Based on the previous available documents involving molecular events during plant somatic embryogenesis, this report aims to review the advances that have been made for the past several years in the area of molecular mec...
Isolation and in silico characterization of full-length cinnamyl alcohol dehydrogenase gene involved in lignin biosynthesis in Neolamarckia cadamba
Cinnamyl alcohol dehydrogenase (CAD) catalyzes the reduction of cinnamaldehyde to ρ-coumaryl, coniferyl, and sinapyl alcohols during the last stage of lignin biosynthesis pathway. The CAD gene expression is believed to b...
Inducible Antimicrobial Compounds (Halal) Production in Honey Bee Larvae (Apis mellifera) from Rumaida, Taif by injecting of various dead Microorganisms extracts
In the current study, the antimicrobial compounds in induced Honey bee larvae (Apis mellifera) from the Taif region were extracted from hemolymph and assigned as Halal safe product. Their minimum inhibitory concentration...