THE COMBINATION THERAPY MODEL OF Andrographis paniculata EXTRACT AND CHLOROQUINE ON Plasmodium berghei INFECTED MICE
Journal Title: Asian Journal of Pharamceutical and Clinical Research - Year 2015, Vol 8, Issue 2
Abstract
 Objective: The aim was to determine the antimalarial drug effectiveness of the combination therapy model of 80% ethanolic extract of sambiloto (EES)and chloroquine on Plasmodium berghei infected mice.Methods: Five groups of P. berghei infected mice were used in this study, divided by three combinations therapy models of EES and chloroquinegroups (Model A, B, C), one monotherapy of EES group (Model D) and one untreated group (Model E) as a control. EES and chloroquine were usedat a dose of 100 mg/kg mice body weight and 0.15 mg/kg mice body weight, respectively. Only for Model C, chloroquine was used at a dose of10 mg/kg mice body weight. Three combinations therapy models consisted of Model A: Infected mice treated by EES and chloroquine for 4 days;Model B: treated by EES for 4 days and chloroquine for 1 day at 1st day; Model C: treated by EES for 4 days and chloroquine for 1 day at 4th day.Meanwhile, Model D and E were treated by EES and vehicle each for 4 days, respectively. Blood smear of all mice was prepared with Giemsa stain.Antimalarial activity was determined by the difference between the mean value of parasitemia of control (Model E, taken as 100%) and those of theexperimental group (Model A, B, C and D).Results: Three combination therapy models (Model A, B, C) and one monotherapy (Model D) were showed antimalarial activities against parasitewith inhibition of parasite growth by 85.61%, 69.31%, 73.64% and 65.14%, respectively.Conclusion: Model A was showed as the most effective combination therapy model on mice infected by malaria. The results indicated that combinationtherapy model of EES and chloroquine were able to increase the antimalarial effectiveness by 85.61% inhibition of parasite growth and having smallerrisk of resistance by using low dose of chloroquine (0.15 mg/kg mice body weight).Keywords: Antimalarial combination therapy, Andrographis paniculata, Chloroquine.
Authors and Affiliations
Achmad Fuad Hafid
Keywords
Related Articles
- EP ID EP605637
- DOI -
- Views 83
- Downloads 0
MORPHOMETRIC STUDY OF PROXIMAL FEMUR AND ITS CORRELATION WITH BONE MINERAL DENSITY. A CROSS-SECTIONAL STUDY
Objective: Proximal femoral morphometries such as hip axis length (HAL), femoral neck (FN) axis length (FNAL), and FN shaft angle (FNSA) are important parameters for prediction of fracture risk. These parameters are affe...
Evaluation of phytochemical constituents, quantitative analysis and antimicrobial efficacy of potential herbs against selected microbes
 Objectives: The aim was to evaluate the physicochemical composition, phytochemical analysis and antimicrobial activity of plant extracts.Methods: The plant samples (Citrus reticulata, Camellia sinensis and Punica grana...
ESTIMATION THE ANTIBACTERIAL EFFECT OF CURCUMIN AND ROSEMARY AMONG PATIENTS WITH DENTAL CARIES IN VITRO
Objective: The aim is to estimate the effect of curcumin and rosemary as antibacterial agents among dental caries cases. Methods: Samples of saliva were randomly collected from 40 patients in Al-Furat General Hospital w...
NOVEL APPROACH OF EXTRACTION AND CHARACTERIZATION OF OKRA GUM AS A BINDER FOR TABLET FORMULATION
Objective: The major objective of the present investigation was to extract a natural polymer (okra gum) with its characterization as pharmaceutical binder and to formulate, develop, and evaluate the compression-coated ta...
ANTIDEPRESSANT-LIKE ACTIVITY OF FLOWERS OF TECOMELLA UNDULATA IN MICE SUBJECTED TO CHRONIC UNPREDICTABLE MILD STRESS
Objective: Flowers of Tecomella undulata have been reported to be a rich source of flavonoids such as rutin and quercetin. The present study was designed to evaluate the effect of ethanol extract of flowers of T. undulat...