The effect of acetone extract of Moringa peregrina on Trichomonas vaginalis, Candida albicans and macrophage cells in vitro
Journal Title: مجله علمي دانشگاه علوم پزشكي كردستان - Year 2018, Vol 23, Issue 93
Abstract
Background and Aim: Vulvovaginal candidiasis and trichomoniasis account for at least 50 percent of all cases of vaginal infections. The main drugs used to treat these infections have numerous side effects and drug resistance to them is on the rise. The aim of this study was to investigate the anti-trichomonas and anti candidal effects of acetone extracts of Moringa peregrina in vitro. Material and Methods: We used acetone extracts of Moringa peregrina at concentrations of 375, 750, …, 3000 and 4000 µg/ml for the treatment of trichomoniasis; and concentrations of 0/003,…, 1 and 2mg/ml for the treatment of candidiasis. We evaluated the effect of the extracts after 24 and 48 hours. The final number of viable parasites were determined by trypan blue staining and neobar lamella; and IC50 (50% Inhibitory Concentration) value was calculated. We also calculated MIC (Minimum Inhibitory Concentration) of the extract for candida. The cytotoxic effect of the extract on the mice macrophage cells was investigated. Result: Comparison between treatment and control groups revealed a significant decrease in the viability of parasites in the treatment group at all concentrations after both 24 and 48 hours (P <0.05). After 24 hours the IC50 and SI values were calculated as 682 and 4.1 for parasite respectively and MIC value was 2 mg/ml for Candida albicans. Conclusion: Considering favorable effects of acetone extract of Moringa peregrina on inhibiting the growth of Trichomonas vaginalis and Candida albicans identification and isolation of active ingredients of the plant, may lead to use of this extract for the treatment of both infections in the future studies.
Authors and Affiliations
B M Ebrahimi, PhD J Sadraei, PhD Sh Roudbarmohammadi, F Nikoomanesh
Keywords
Related Articles
- EP ID EP283371
- DOI -
- Views 88
- Downloads 0
Isolation, morphological characterization and host range determination of Iranian bacteriophages against Salmonella reference collection
Background and Aim: Salmonella spp. is important in medicine. Most related infections are self-limited. Antibiotics are used for high risk patients such as children, old individuals and immune-compromised patients. Overu...
The effect of endurance activity on miR-499 and sox6 genes expression in fast and slow twitch skeletal muscles
Background and Aim: Endurance activity can affect function and also factors involved in skeletal muscle transcription. The aim of this study was to investigate the effects of an endurance activity program on the Sox6 gen...
Assessment of urban air quality in Sanandaj city and attribution of cardiovascular- respiratory diseases, and preterm birth in 2015-16
Background and Aim: Over the past decades, air pollution in the urban areas has shown an increasing trend. Air pollutants have resulted in different diseases. The aim of this study was to study the air quality in Sananda...
Effects of vitamin D supplementation and resistance training on insulin resistance, lipid profile and body fat percentage in T2D men with vitamin D deficiency.
Background and Aim: The aim of this study was to investigate the effects of vitamin D supplementation during progressive resistance training on insulin resistance, blood lipids and body fat percentage in T2D men with vit...
Protective effects of hydro-alcoholic garlic extract on spermatogenic disorders in streptozotocin-induced diabetic C57BL/6 mice
Background and Aim: Diabetes can cause sexual dysfunction including spermatogenic disorders in the diabetic patients. The aim of the present study was to investigate the antioxidant effects of garlic extract in diabetic...