The effect of DEMI-001 isolate of Metarhizium anisopliae on Aphis Rose (Hemiptera: Aphididae) at different temperatures
Journal Title: International Journal of Farming and Allied Sciences - Year 2015, Vol 4, Issue 6
Abstract
Rose Aphid Macrosiphum rosae L. is one of most important pests of rose which reducing the quality and quantity of products by attacking and hurting the leaves and branches. In this study, the effect of DEMI-001 isolate of Metarhizium anisopliae was evaluated on mortality of aphids, at various temperatures, 10, 15 and 20°C. Ten adult aphids were placed on rose leaves in a treatments in three replications. Conidia of M. anisopliae sprayed on leaves of rose at concentrations of 104, 105 and 106 spores/ml. According to the results of this experiment, temperature and concentrations of conidia suspension had the significant effect in all treatments. Accordingly, at 10°C, 24 hours after inoculation, 40.5% and 6% mortality was observed for concentration of 106 and control treatment, respectively. After 48 hours, at concentration of 106 and the temperatures of 10 and 20°C, mortality was 52.1% and 74.2%, respectively. In the same condition, mortality of the control treatment was 5.7% and 22.1%, respectively. Given the significant differences among the control and other treatments, recommended the use of this fungus against black bean aphid in the greenhouse and field conditions.
Authors and Affiliations
Batol Jafarinasab
Keywords
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