THE EFFECT OF HEAT STRESS ON HYPERHYDRICITY AND GUAIACOL PEROXIDASE ACTIVITY (GPOX) AT THE FOLIAR LAMINA OF SEDUM TELEPHIUM L. SSP. MAXIMUM (L.) KROCK. VITROPLANTLETS
Journal Title: Analele Stiintifice ale Universitatii Alexandru Ioan Cuza din Iasi, Serie noua, Sectiunea II a. Biologie vegetala - Year 2014, Vol 60, Issue 2
Abstract
Heat stress causes an array of physiological, biochemical and morphological changes in plants, affecting plant growth and development. In order to confirm the connection between oxidative stress in plants and hyperhydricity (vitrification), we used a Sedum telephium ssp. maximum plant as a test material. The changes of the antioxidant enzyme guaiacol peroxidase (GPOX) activity in in vitro Sedum plantlets were studied under increased temperatures and compared with in vitro plantlets kept at 21ºC as the control. The results showed that the highest activity of GPOX was in response to heat stress of 41°C on day 10 after treatment (102,32 UI/mL), compared with the control where the GPOX activity remained unchanged (38,1 UI/mL) until the end of the experiment (40 days). In case of plants subjected to heat stress starting from the first days of vitroculture, the vitrification process has begun and with increasing of GPOX activity, vitrification was becoming more intense. However, the vitroplantlets grown for 40 days under constant temperature showed no signs of vitrification. Cytological studies of the hyperhydric foliar lamina were also performed at the end of 40 days of the experiment. Microscopic studies have shown that the hyperhydric mesophyll cells of S. telephium ssp. maximum are in a process of necrosis, disorganization, extremely low on cytoplasm, have an imploded cellular content and big gaps between them. The results from this study suggest that the heat stress plays a role in triggering the vitrification process in the in vitro micropropagated plants.
Authors and Affiliations
MIRELA ARDELEAN, DORINA CACHIŢĂ-COSMA, AUREL ARDELEAN, CALIN LADAŞIU, VALENTIN MIHALI
Keywords
Related Articles
- EP ID EP163333
- DOI -
- Views 78
- Downloads 0
THE ANALYSIS OF THE VASCULAR FLORA FROM THE MOLDOVIŢA’S RIVER BASIN (SUCEAVA COUNTY)
This paper analyses the vascular flora from the Moldoviţa’s River Basin, in the aspect of the participation of different life form categories, floristic elements, also from the point of view of the ecological prefe...
STUDIES CONCERNING THE HISTO-ANATOMY AND BIOCHEMISTRY OF MENTHA LONGIFOLIA (L.) HUDS. DURING VEGETATIVE PHENOPHASE
The article analyzed the histo-anatomy of the vegetative organs of species Mentha longifolia (L.) Huds collected from Negreşti (Vaslui). On the surface of the vegetative organs was identified the presence of tector...
MORPHO-ANATOMICAL CONSIDERATIONS UPON THE SHOOT OF SOME ROSA L. CULTIVARS FROM THE BOTANIC GARDEN OF IASI (2nd NOTE)
The paper is focused on a comparative study regarding shoot morphology and anatomy of two climbing rose cultivars, from the Botanic Garden of Iasi: ‘Ilse Krohn Superior’ and ‘Sympathie’. The shoot presents a thick collen...
CONTRIBUTIONS TO THE STUDY OF FLORA AND VEGETATION FROM BASIN VALEA MORISCA
This paper contains preliminary results obtained in the period 2009-2010, materialized in flora conspect, cenotaxonomic conspect and analysis of aquatic and marsh flora in the territory Valea Morisca. The flora con...
NEW CONTRIBUTIONS TO THE STUDY OF THE ASSOCIATIONS FROM QUERCO – FAGETEA BR. – BL. ET VLIEGER IN VLIEGER 1937 CLASS, FROM THE LOWER BASIN OF THE BISTRIŢA RIVER
The paper presents the association Galio schultesii – Fagetum (Burduja et al. 1974) Chifu et Ştefan 1994 from Querco – Fagetea Br. – Bl. et Vlieger in Vlieger 1937 and its three sub-associations (fagetosum, quercetosum d...