The effect of human amniotic epithelial cells on urethral stricture fibroblasts
Journal Title: Journal of Clinical and Translational Research - Year 2019, Vol 5, Issue 1
Abstract
Background: Urethral stricture disease (USD) is effectively managed by buccal mucosa (BM) urethroplasty. Lack of adequate healthy BM has led to the use of autologous tissue-engineered BM grafts. Such grafts are costly, not easily scalable and recurrence of the stricture is still a problem. Hence, there is a requirement for cost-effective, scalable cells with innate antifibrotic properties which seem to be fulfilled by human amniotic epithelial cells (HAMECs). The effect of HAMECs on USD is unknown. Aim: To study the effect of HAMECs-CM on human urethral stricture fibroblast (USF) cells by using in-vitro migration assay and molecular techniques. Materials and Methods: USF cells were derived from six patients undergoing urethroplasty. HAMECs were derived from one placenta after delivery. The effect of HAMECs-CM on USF cell migration was observed using a standard in vitro scratch assay over a period of 3 days. The effect of HAMECs-CM on the expression levels of markers alpha-smooth muscle actin (α-SMA) and tissue inhibitor of metalloproteinases (TIMP-1) in USF cells was also examined. Results: The HAMECs-CM suppressed the migration of USF cells in in vitro scratch assay. The HAMECs-CM consistently downregulated α-SMA, but not TIMP-1. Conclusions: HAMECs have shown antifibrotic activity on USF cells in this in vitro study. Relevance for Patients: HAMECs could serve as an alternative cell source for tissue-engineered urethroplasty.
Authors and Affiliations
Sanjay Gottipamula, Sudarson Sundarrajan, Kumar Chokalingam, K. N. Sridhar
Keywords
Related Articles
- EP ID EP678744
- DOI -
- Views 240
- Downloads 0
FXR agonism protects against liver injury in a rat model of intestinal failure-associated liver disease
Background: Intestinal failure-associated liver disease (IFALD) is a clinical challenge. The pathophysiology is multifactorial and remains poorly understood. Disturbed recirculation of bile salts, e.g. due to loss of bil...
Cardiac vagal activity and daily clinical practice
During most of the day heart rate (HR) is dominated by cardiac vagal (parasympathetic) activity – a fact taught in physiology classes and duly repeated at the appropriate exams. Only later in medical practice does one b...
How the vagus nerve produces beat-to-beat heart rate variability; experiments in rabbits to mimic in vivo vagal patterns
Background and Aim: Analysis of heart rate variability (HRV) has recently become the playing field of mathematicians and physicists, losing its relation to physiology and the clinic. To set the record straight, a set of...
Legal challenges for the implementation of advanced clinical digital decision support systems in Europe
Systems based on artificial intelligence and machine learning that facilitate decision making in health care are promising new tools in the era of ‘personalized’ or ‘precision’ medicine. As the volume of patient data and...
Connexin-based signaling and drug-induced hepatotoxicity
Being critical mediators of liver homeostasis, connexins and their channels are frequently involved in liver toxicity. In the current paper, specific attention is paid to actions of hepatotoxic drugs on these communicati...