THE EFFECTIVENESS OF DISINFECTION METHODS ON GERMINATION OF GOJI SEEDS (Lycium barbarum L.) IN IN VITRO CULTURE
Journal Title: Folia Pomeranae Universitatis Technologiae Stetinensis Agricultura Alimentaria Piscaria et Zootechnica - Year 2017, Vol 43, Issue 336
Abstract
Using disinfectant components is one of main ways to control of fungal and bacterial contaminations in the in vitro culture. Alternative to commonly used chemicals is ozonation. The present study was focused on the efficiency of seeds disinfection of two goji cultivars ‘A’ and ‘New Big’ using eight different treatments, 7%, 10% and 15% NaOCl and 0.2% HgCl2 for 15 minutes each, O3 gas and O3 in water for 5 and 15 minutes. The highest percentage of germinated seeds of ‘A’ and ‘New Big’ (respectively 62% and 78%) with the lowest number of infections (respectively 8% and 14%) was obtained after using 7% NaOCl solution. Less effective was 0.2% HgCl2 solution, where the percentage of germinated seeds of ‘A’ and ‘New Big’ was lower (respectively 37% and 30%). However, after O3 in water treatment of goji ‘A’ and ‘New Big’ for 15 minutes the percentage of germinated seeds was the highest (respectively 47%, and 53%) and sterility of the cultures of goji ‘A’ was 100%. In addition to, the ozonation of the seeds showed similar disinfection efficiency, as with 15% NaOCl solution. Therefore, ozone gas makes an alternative for commonly used disinfectants.
Authors and Affiliations
Arleta KRUCZEK, Marcelina KRUPA-MAŁKIEWICZ, Ireneusz OCHMIAN
Keywords
Related Articles
- EP ID EP411727
- DOI -
- Views 75
- Downloads 0
FODDER CROPS AS PROMISING SOURCES OF BIOLOGICALLY ACTIVE COMPOUNDS IN INDUSTRIAL LIVESTOCK BREEDING
The content of isoflavonoids in the green mass of the leading Ukrainian soybean varieties by the vegetation stages were determined, as well as the presence of a series of phenolic compounds in different vegetative organs...
INFLUENCE OF THE DEGREE OF EJACULATE DILUTION ON THE RESULTS OF THE ASSESSMENT OF MOTILITY OF SPERM OF BREEDING BULLS OBTAINED BY TWO METHODS
The experiment carried out aimed to examine the effectiveness of two methods of assessment of the parameters of sperm motility in thawed semen depending on the degree of its dilution. The semen was collected from 12 Simm...
CHARACTERIZATION OF SELECTED TECHNIQUES OF MACERATION BONES OF GALLUS GALLUS DOMESTICUS
Five carcasses of domestic hens Gallus gallus domesticus were starting material for this study. Three carcasses were thermally treated to obtain all parts of skeleton. One set of initial material was placed in water solu...
EPIGENETICS OF REPRODUCTION
Epigenetic research offers a significant opportunity to understand the role of environmental interactions on the genome and disease, while enabling modulation of these interactions in order to improve human health. Knowl...
VARIABILITY OF THE INCIDENCE OF Fusarium SPECIES AND MYCOTOXINS IN THE GRAIN OF WHEAT, DEPENDING ON SOIL TILLAGE SYSTEM AND CULTIVAR
The aim of the present work was to analyse the variability of the incidence of Fusarium species and mycotoxins in the grain of wheat, depending on wheat cultivar and soil tillage system. The experimental factors included...