THE EFFECTIVENESS OF SOURSOP LEAF EXTRACT AGAINST GROWTH OF AGGREGATIBACTER ACTINOMYCETEMCOMITANS ATCC® 6514™ IN VITRO
Journal Title: Asian Journal of Pharamceutical and Clinical Research - Year 2018, Vol 11, Issue 12
Abstract
Objective: The objective of this study was to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) antibacterial power of soursop leaf extract on Aggregatibacter actinomycetemcomitans (Aa) ATCC® 6514™ growth.Methods: This study was experimental laboratory with post-test only control group design and consists of 8 treatment groups that were soursop leaf extract group with concentration 50%, 25%, 12.5%, 6.25%, 3.125%, and 1.5625% as well as negative control groups were brain heart infusion broth (BHIB) media and chlorhexidine as positive controls. Each treatment was done 3 repetitions. Testing the effectiveness of soursop leaf extract using dilution methods on BHIB and subculture media on Mueller Hinton Agar (MHA) media. The number of Aa ATCC® 6514 ™ colonies was calculated manually using the total plate count method on the MHA media. Data were analyzed using Kruskal–Wallis test (p<0.05) followed by least significance different (LSD) test to see the significant mean difference between treatment groups.Results: Concentration of MIC from soursop leaf extract on Aa ATCC® 6514™ growth was 1.5625% and MBC was 6.25%. LSD assay results showed significant difference effect (p<0.05) Aa ATCC® 6514™ from each treatment group.Conclusion: Soursop leaf extract has antibacterial effectivity against Aa ATCC® 6514 ™.
Authors and Affiliations
Ameta Primasari, Minasari Nasution, Nurul Hidayati Arbi, Dini Permata Sari, Mohammad Basyuni
Keywords
Related Articles
- EP ID EP605237
- DOI 10.22159/ajpcr.2018.v11i12.28435
- Views 139
- Downloads 0
INVITRO ANTIOXIDANT ACTIVITIES OF VARIOUS LEAVES EXTRACTS FROM FIVE VARIETIES OF RAMBUTAN (Nephelium lappaceum) AND ITS CORRELATION WITH TOTAL FLAVONOID, PHENOLIC, CAROTENOID CONTENT
 Objectives: The objectives of this research were to study antioxidant activity from different polarities extracts of rambutan (Nephelium lappaceum) leavesusing two methods of antioxidant testing which were 2-2-diphenyl...
BIOMEDICINAL AND GASTROPROTECTIVE ASPECTS OF ORGANOBISMUTH COMPOUNDS: RECENT APPROACHES AND FUTURE PERSPECTIVES
Objective: Peptic ulcer is a disease caused due to an imbalance of aggressive and defensive factors in the body. Helicobacter pylori infections and colonization are also associated strongly to the peptic ulcer disease. B...
IN VITRO-IN VIVO CORRELATIONSHIP APPROACH OF THE PREPARED MAGNETIC MICROSPHERES OF CYTARABINE
Objective: The objective of this study was to study the in vitro-in vivo correlationship between the magnetic microspheres prepared by continuous solvent evaporation (CSE) method by 32 factorial designs. Methods: CSE te...
PREDICTORS ASSOCIATED WITH ADVERSE DRUG REACTIONS AMONG GERIATRIC PATIENTS IN AN OUTPATIENT CLINIC
Objective: The process of aging involves an individual’s structural and functional depravity of their organ systems often results in compromised pharmacological principles of the prescribed drugs. Multimorbidity and poly...
ANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF NEW STABILITY-INDICATING REVERSE-PHASE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY METHOD FOR SIMULTANEOUS ESTIMATION OF METFORMIN HYDROCHLORIDE AND EMPAGLIFLOZIN IN TABLET DOSAGE FORM
Objective: The objective of this study was to develop and validate a stability-indicating reverse-phase high-performance liquid chromatography (RP-HPLC) method for the simultaneous estimation of the metformin and empagli...