The effects of cynodon dactylon on the immune response of NMRI-MICE after challenge with REV1
Journal Title: International Journal of Medical Research & Health Sciences (IJMRHS) - Year 2016, Vol 5, Issue 10
Abstract
Cynodon dactylon is used in Iranian traditional medicine as a healing agent for reducing the complications of diabetes mellitus. We proposed that Cynodon dactylon may perform its effects through moderating humoral and cellular immune responses. We aimed to determine the possible effects of hydroalcholic extract of Cynodon dactylonon humoral and cellular immune responses following the Rev1 challenge in the mouse model. 20 NMRImalemice were randomly grouped in two equal groups and immunized with Rev1[0.1 ml Rev1+0.9 PBS[. Mice in the treatment group orally received 400 mg/kg hydroalcoholic extract of Cynodon dactylon every day from the beginning of the study for 2 weeks. Blood samples were obtained from the animals 5 days after the last injection. Moreover, 48 hr before bleeding time, Rev1[0.1 ml Rev1+0.9 PBS[was injected into the left hind foot pad of mice. The levels of anti-Rev1 antibody and the specific cellular immune responses were measured by microhemagglutination test and footpad thickness, respectively. Moreover, susceptibility of macrophages respiratory burst and proliferation of immune cells were measured in order withNitroblue tetrazolium[NBT] and Microculture Tetrazolium Assay [MTT]. The concentrations of IL-1, TNFα, Il-6, and IL-10 in the serum were determined using commercially available ELISA kits. We found a significant increase in anti-Rev1 antibody levels and simultaneously a significant decrease in the level of cellular immunity[DTH] in the treatment group compared to the control group. Lymphocyte proliferation index in splenocytes was significantly increased in the treatment group. However, the level of respiratory burst in phagocytic population of splenocytes dramatically decreased in the treatment group compared to the control. A significant decrease in IL-6, TNF-α , IL-1 and increaseIl-10 serum levels were also seen in the treatment group. Cynodon dactylon extract could have an anti-inflammatory effect through downregulation of pro-inflammatory cytokines and can be considered as the moderating immune system compound.
Authors and Affiliations
Behrooz Ilkhanizadeh| Department of Pathology, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran, Seyed Jalil Mousavi| Diseases Specialist, Department of Infectious Diseases, Urmia University of Medical Sciences, Urmia, Iran, Masoumeh Pourjabali| Department of Pathology, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran, Leila Zarei| Solid Tumor Research Center, Urmia University of Medical Sciences, Urmia, Iran Corresponding E mail: leilazarei652@yahoo.com
Keywords
Related Articles
- EP ID EP12103
- DOI -
- Views 323
- Downloads 12
GENDER DIFFERENCES IN AUTONOMIC FUNCTIONAL STATUS IN RESPONSE TO STRESS
Introduction: Men and women are similar in their cognitive appraisal of a stress. But their behavior is different when exposed to stress. As stress responses and cognitive abilities are closely associated with autonomi...
A comparative profile analysis of neuropsychological functioning in patients with schizophrenia and bipolar and normal group based on stroop test and Benetton visual retention test
The relation between brain and mind is quite evident. Neuropsychological instruments have made considerable contributions in evaluating cognitive functions among healthy individuals and those patients suffering from ps...
Molecular Identification of Cholesterol Oxidase Enzyme-Producing Streptomyces Bacteria in Soil of Lorestan Province, Iran
Bacterial Cholesterol oxidase (CHO) is a monomeric, bi-functional and Flavin adenine dinucleotide-linked enzyme that catalyzes oxidation and isomerization cholesterol in the presence of molecular oxygen. Recently, power...
PANCREATOBILIARY VERSUS INTESTINAL TYPE OF HISTOLOGICAL DIFFERENTIATION: A COMPARATIVE STUDY WITH HISTOMORPHOLOGICAL PROGNOSTIC FACTORS IN PERIAMPULLARY CARCINOMA
Objectives: Periampullary carcinoma is a term widely used to define a heterogenous group of neoplasms arising from the head of the pancreas, ampulla of Vater, terminal common bile duct and the duodenum. Periampullary c...
COMPARISON OF POST OPERATIVE PULMONARY FUNCTION BETWEEN OPEN SURGERIES AND LAPAROSCOPIES
Background: Respiratory function is depressed after abdominal surgery. Less trauma to the abdominal wall results in early postoperative recovery. Aim: The study was planned to compare recovery of postoperative respira...