The effects of insulin on the expression levels of ADAMTS6 & 19 in OUMS-27 cell
Journal Title: Dicle Tıp Dergisi - Year 2014, Vol 41, Issue 3
Abstract
Objective:A Disintegrin-like Metalloproteinase with Thrombospondin Motifs (ADAMTS) proteins are kind of matrix metalloproteinase enzymes that primarily founds in the extracellular matrix (ECM). Insulin is an important anabolic hormone, which acts on many tissues. The aim of this study is to evaluate the time-dependent effects of insulin on the two functionally unknown enzyme expressions (ADAMTS6 & 19) in OUMS-27 human chondrosarcoma cell line. Methods: OUMS-27 cells were cultured in Dulbecco’s modified Eagle’ medium (DMEM) alone and DMEM containing 10 μg/mL insulin. The medium was changed every other day up to 11th day. Cells were harvested at 1, 3, 7, and 11th days and RNA isolation was performed at appropriate times according to study setup. The levels of RNA expression of ADAMTS6 and 19 were estimated by qRT-PCR using appropriate primers. Results: According to qRT-PCR analysis, ADAMTS6 mRNA expression was found to be decreased as early as one day after insulin application and continued up to day 11, the last day of insulin induction (p=0.008). The ratio of ADAMTS6 in insulin-applied groups was changed between 1/2 and 1/4 of control values. The changes in ADAMTS19 mRNA levels in insulin-applied cells were not statistically significant compared to control cell group. Conclusion: Our results demonstrated that insulin has a potential effect on lleviation of loss of extracellular matrix compounds by diminishing ADAMTS6 levels. To test this hypothesis and finding, more investigations are needed to recognize the real functions of orphan ADAMTS proteins. Key words: Insulin, ADAMTS6, ADAMTS19, chondrosarcoma, OUMS-27, RNA
Authors and Affiliations
Veli Uğurcu, Sümeyya Akyol, Aynur Altuntaş, Rıdvan Fırat, S. Fatih Kurşunlu, Özlem Çakmak, Kadir Demircan
Keywords
Related Articles
- EP ID EP158057
- DOI 10.5798/diclemedj.0921.2014.03.0453
- Views 112
- Downloads 0
The frequency of gestational diyabetes mellitus in a maternity hospital antepartum clinic
The aim of this study was to determine gestational diabetes mellitus (GDM) frequency and age related frequency with GDM screening tests in patients whom referred to our hospital’s antepartum clinic for routine follow-up....
Perkütan endoskopik gastrostomi uyguladığımız hastalarda uzun dönem sonuçlarımız
Amaç: Oral gıda alamayan, özellikle yoğun bakım ünitelerinde yatmakta olan hastalara enteral beslenme desteği sağlamak amacıyla minimal invaziv bir metod olan perkutan endoskopik gastrostomi (PEG) deneyimimizi sunmayı...
Examining the expression level of VEGF and vimentin by immunohistochemistry and Western Blot in preeclamptic and normotensive placentas
Objective: In this study, we aimed to investigate the expression levels of vascular endothelial growth factor (VEGF) which stimulates angiogenesis and vimentin, an intermediate cytoskeleton filaments, in both preeclampt...
A Comparison Of Mini-incisional Ligation, Laser Ablation And Cyanoacrylate Embolization For Incompetent Perforating Vein Closure
Objective: Our aim was to compare the short- and the midterm results of three main methods of incompetent perforating vein treatment. Methods: Data of 174 procedures performed between January 2012 and January 2015, in...
Çalışan kalpte koroner baypas cerrahisi sırasında trimetazidine ve glikoz-insülin-potasyum kullanımının miyokard üzerine etkileri
Amaç: Çalışan kalpte koroner baypas esnasında trimetazidine ve glikoz - insülin - potasyum (GİK) solüsyonunun miyokardiyal iskemi ve reperfüzyon hasarı üzerine etkilerini araştırmak üzere ileri dönük, randomize, kontroll...