The Effects of miR-30a and miR-17 Biomarkers and Methylprednisolone in Experimental Spinal Cord Injury in Rats
Journal Title: Acta Medica Ruha - Year 2023, Vol 1, Issue 3
Abstract
Introduction: Methylprednisolone, is a neuroprotective steroid with many effective mechanisms such as inflammation, cell blood flow changes, and apoptosis in the early period following spinal cord injury. This study aimed to demonstrate the inhibitory activity of methylprednisolone to prevent early injury through microRNA expressions, which are predicted to play a role in genomic regulation.Method: This present study was conducted on 56 male Sprague-Dawley rats. All the animals divided into 8 groups which consists of 7 animals each. Laminectomy procedure was performed between levels T5-8. All the groups except the two control groups have been damaged with the Yasargil aneurysm clip for 1 minute at the T5 level. T5-8 spinal cord tissue was removed at the 6th, 12th, and 24th hours after clipping. Methylprednisolone was given to the intraperitoneal cavity only to the clipped groups. As a result of histopathological and immunohistochemical examination, there was a significant decrease in cell necrosis, edema, hemorrhage and white matter-gray matter transition in groups given methylprednisolone. Damaged spinal cord samples excised from all rats. miR-30a and miR-17 gene expression levels were evaluated by quantative PCR method.Results: miR-30a was significantly upregulated at 12th and 24th hours after spinal cord injury and this rise was restricted in the methylprednisolone treated groups.. miR-17 was down-regulated at the 6th hour and and reached its lowest level at the 12th hour.Conclusion: Methylprednisolone has statistically significant healing effects on spinal cord injury through the mechanism of miR-30a and miR-17.
Authors and Affiliations
Ece Uysal, Hidayet Safak Cine, Suat Erol Celik
Keywords
Related Articles
- EP ID EP722151
- DOI 10.5281/zenodo.8325077
- Views 22
- Downloads 0
Diyabetik ve Nondiyabetik Koroner Arter Hastalarında Kardiyopulmoner Bypass Öncesi ve Sonrası Troponin-I Düzeylerinin Karşılaştırılması - Comparison of Troponin-I Levels Before and After Cardiopulmonary Bypass in Diabetic and Nondiabetic Coronary Artery Patients
Giriş: Kardiyak troponin I (cTnI) miyokardiyal hasar için tanısal bir biyobelirteçtir.Amaç:Bu çalışmanın amacı diyabetik ve nondiyabetik koroner arter hastalarında KPB öncesi ve sonrası Troponin-I düzeylerini...
Effect Of Epidermal Growth Factor & Platelet-Rich Plasma In Experimental Rat Burn Model
Introduction:Today, drugs are being tried with various methods for the early healing of burn wounds. However, no drug still shows the desired effect in burn healing.Objective:This experimental study investig...
ClosureMethodsforthePreventionofCSFFistulainTranssphenoidalSurgery:ALargeStudy
AimandBackground:AimandBackground:TheprimaryobjectiveofthisextensiveseriesistoconductacomparativeanalysisofmicroscopesandendoscopesandinvestigatetheextentofvariabilityinCSFleakagebasedonthesurgicalapproachemployed,theocc...
Symptomatic And Radiological Evaluation Of Patients Undergoing Surgical Treatment And Follow-Up With The Diagnosis Of Sylvian Arachnoid Cyst
Objective: Arachnoid cysts are the most common intracranial cysts, constituting 1% of all intracranial space-occupying lesions. In this research, we aimed to analyze the treatment process, radiological findin...
Biochemical and Histopathological Evaluation of The Effect of Thiamine Pyrophosphate on Favipiravir-İnduced Degenerative Corneal and Scleral Damage in Rats
Introduction:Favipiravir causes ocular toxicity at high doses. Thiamine pyrophosphate (TPP) therapy can prevent ocular damage by reversing oxidative damage.Objective:To investigate the ocular effect of favipira...