The efficacy of polyherbal formulation of Moringa oleifera, Viola odorata, Allium sativum against microbes- synergistic effect
Journal Title: Indian Journal of Pharmaceutical and Biological Research - Year 2015, Vol 3, Issue 4
Abstract
The purpose of our present study is to test antimicrobial and antifungal activity of polyherbal formulations. The testing of the antimicrobial activity on polyhernal formulation was done against gram positive (Staphylococcus aureus, Bacillus subtilis) and gram negative (Salmonella typhi, Escherichia coli,) bacteria. The testing of antifungal activity was done against Aspergillus niger and Candida albicans with different combinations of polyherbal formulation. The microorganism to be tested was grown in relevant nutritional media. The solvent used for the extraction of Moringa oleifera, Viola odorata, Allium sativum was methanol. These methanolic extracts of chosen plants were further used in specific proportions for the preparation of polyherbal formulation (PF-1, PF-2, and PF-3). The resultant compositions were further used to check the efficacy against selected experimental microorganisms. PF-3 showed the significant high zone of inhibition against all the experimental microorganisms as compared to the other two combinations PF-1 and PF-2. Thus, the result has revealed that the antimicrobial activity is due to the synergistic effect of secondary metabolites present in these selected plants.
Authors and Affiliations
Meenakshi P Singh| Department of Pharmacognosy & Biotechnology, East Point Research Academy, Bangalore- 560049, Karnataka, INDIA, Jagadeesh Singh SD| Department of Pharmacognosy & Biotechnology, East Point Research Academy, Bangalore- 560049, Karnataka, INDIA, Rajesh R| Department of Biotechnology, Tumkur University, Tumkur- 572106, Karnataka, INDIA
Keywords
Related Articles
- EP ID EP15483
- DOI -
- Views 287
- Downloads 15
Development and Validation of Stability indicating method for the estimation of Axitinib in tablet dosage forms by UPLC
A Stability Indicating Ultra-Performance Liquid Chromatography method was developed and validated for quantification of Axitinib in tablets. The chromatographic separation was done in an isocratic mode using the STD RP-1...
Evaluation of Vitamin B1, B2 and B6 Tablets in Bangladesh by UV-Vis Spectrophotometer
Thiamine hydrochloride (vitamin B1), riboflavin (vitamin B2) and pyridoxine hydrochloride (vitamin B6) were assayed in pharmaceutical dosages form. Thiamine hydrochloride of 100 mg dose of six different companies, ribofl...
Allium Cepa Root Chromosomal Aberration Assay: A Review
Higher plants, an important material for genetic tests to monitor various pollutant present in the environment. Among the plant species, Alium cepa has been used to evaluate chromosome aberrations and disturbances in th...
Indigenous uses of plants in different women ailments in Garhwal region
A survey of villages of Garhwal region was done to identify medicinally important plants used by local peoples of that region against different women ailments. Paper deals with 67 plant species of 42 families as a herb...
Antibacterial activity of Crotalaria pallida Aiton. (Fabaceae)
Crotalaria pallida Aiton. Fabaceae has been used for the treatment of various diseases like. The aim of the present study is to assess antibacterial activity of methanolic leaves extract of Crotalaria pallida Aiton. Es...