THE EFFICIENCY OF GROWING ANABAENA HASSALII (KUTZ.) WITTR. UNDER DIFFERENT CULTIVATION CONDITIONS
Journal Title: Naukovyi visnyk Chernivetskoho universytetu. Biolohiia (Biolohichni systemy) - Year 2014, Vol 6, Issue 2
Abstract
This paper is devoted to the estimation of cultivation efficiency of the unialgal culture Anabaena hassalii (Kutz.) Wittr. on the different kinds of medium. The productivity of culture was analyzed by the accumulation of protein and photosynthetic pigments (chlorophyll a and carotenoids). The investigated algae was grown on the Fitzgerald’s artificial medium number 11 in the modification of Zehnder and Gorham and on the water from RAS (Recirculation Water System), that was standardized with the indicators pH and total mineralization. The efficiency of A. hassalii cultivation on the waste water did not differ from the efficiency of cultivation on artificial medium. During the cultivation on both investigated media it was noted the constant increase of algae biomass, that has reached its maximum on the 40-th day. The similar tendencies were set also during the study of total protein content dynamics and chlorophyll a dynamics. The culture of A. hassalii was characterized with maximum content of total protein, that was within 22-23% on the 40-th day of cultivation. It was also observed a gradual increasing of the number of chlorophyll a, which have reached the level of 17-21 mg / g of the dry biomass till the 30-th day of cultivation inclusive on both culture media. It was noticed a linear accumulation of carotenoids in the cells of A. hassalii during the whole period of cultivation. The maximum content of carotenoids is observed in the stationary growth phase of culture A. hassalii on both nutrient media. The cultivation of microalgae A. hassalii on the Fitzgerald’s medium and on the water from RAS has similar growth rates and allows to get the actively growing culture that is characterized by constant increase of biomass, high content of total protein and major photosynthetic pigments. The resulting so productive culture can be used for further processing or can continue to cultivate of the fresh culture medium.
Authors and Affiliations
Л. ЧЕБАН, І. МАЛІЩУК, В. Р. ЛИСАК, М. М. МАРЧЕНКО
Keywords
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