The evaluation of P16 in chronic myeloid leukemia patients
Journal Title: Khoon - Year 2018, Vol 15, Issue 3
Abstract
The evaluation of P16 in chronic myeloid leukemia patients Barazandeh-Rokh M.1, Nasoohi N.1, Molaei M.2, Fallah P.2 1Faculty of Advanced Sciences and Technology, Pharmaceutical Sciences Branch, Islamic Azad University (IAUPS), Tehran, Iran 2Allied-Health Faculty, Alborz University of Medical Sciences, Karaj, Iran Abstract Background and Objectives Chronic Myeloid Leukemia (CML) is a clonal disorder of the hematopoietic stem cell caused by the BCR-ABL receptor tyrosinkinase. The tumour suppressor gene of P16 plays the key role in the cell cycle control. The inactivation of this gene is associated with tumorgenesis, pathogenesis and progression of leukemia. The aim of this study was to evaluate the expression of P16 gene in patients with chronic myeloid leukemia for the identification of the gene expression changes so that the role of the gene in diagnosis and treatment of the disease could be determined. Materials and Methods In this descriptive study, the expression of P16 gene in 40 CML patients in chronic phase and 8 healthy subjects as the control all in Payvand Medical Laboratory was examined by Real Time PCR technique. Results were analyzed using the Relative Expression Software Tool (REST2009). Results P16 gene expression was significantly down-regulated in 32 patients (p ≤ 0.05), and up-regulated in 8 patients (p ≤ 0.05) compared to the control group. Conclusions In conclusion, downregulation or upregulation of P16 may be of importance in the biology of CML.Other studies are also required to elucidate the exact function of this tumor suppressor in cancer. Key words: Myeloid Leukemia، Chronic, PCR, Hematopoietic Stem Cells Received: 1 Sep 2017 Accepted: 20 May 2018 Correspondence: Fallah P., PhD of Hematology & Blood Banking. Allied-Health Faculty, Alborz University of Medical Sciences. Postal Code: 3147734568, Karaj, Iran. Tel: (+9826) 34349800; Fax: (+9826) 34349802 E-mail: parvizfallah@gmail.com
Authors and Affiliations
M. Barazandeh-Rokh, N. Nasoohi, P. Fallah
Keywords
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