The Expressions of CD4+ and CD8 + in Biliary Atresia Mice Model after Exposure with Rhesus Rotavirus (RRV)
Journal Title: Journal of Medical Science And clinical Research - Year 2015, Vol 3, Issue 6
Abstract
Background: Biliary atresia (BA) causesmore than 95% chronic liver disease or cirrhosis and 50-60% liver transplantation. The pathogenesis is unclear.It is proposed that viral infection of the bile duct epitheliumcausing progressive autoreactive inflammation mediated by autoimmune process, resulting fibrosis and bile duct obstruction. Objective: Determine the effect of induction and duration of illness after rhesus rotavirus (RRV) exposure to expression of CD4+ and CD8+ in mice models BA. Methods:Experimental research using 48 babiesborn from 20 mice balb/c, randomized into two groups, placebo (buffered saline) and 1.5 x 106 pfu RRV intraperitoneally in the first 24 hours antenatally. Each group was terminated on 3rd, 7th, 14th and 21st day, then CD4+ and CD8+ expression was measured by flowcytometry. Statistical analysis performed using independent sample t-test, Mann-Whitney and Kruskal-Wallis. Results: There were an increase of CD4+ and CD8+ expression in intervention group compared to control group (p<0.05), except CD4+ in day 3rd (p=0.493), showed the influence of the RRV induced. Influence of interaction duration of illness and RRV induction was also found statistically significant in mice models of BA (p<0.001). The increase of CD4+ and CD8+ expression was noted after 7thday, peaked at 14th day, and decreased at 21st day. Conclusions:RRV induction and duration of illness improves the adaptive cellular immune responses as reflected by the increased expression of CD4+ and CD8+ in mice models BA
Authors and Affiliations
Bagus Setyoboedi
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