The influence of silicon nanoparticles on Mentha piperita rhizogenesis under in vitro conditions
Journal Title: «Таврійський науковий вісник» - Year 2016, Vol 96, Issue
Abstract
The study investigates the influence of silicon nanoparticles in nutrient medium on rhizogenesis of Mentha piperita L. explants in vitro conditions and on further regenerants adaptation in vivo based on the isolated tisssues and bodies culture method. Silicon nanoparticles addition to the nutrient medium for rhizogenesis intensifies the beginning of root formation; microplants form simultaneously 5-6 radiculars. Optimum silicon nanoparticles concentration in nutrient medium for rhizogenesis is determined. By the 28th day of cultivation, in Mentha piperita of Lydia variety the average length of roots had increased by 84 % (р <0.05), of Mama variety by 107 % (р <0.05), and of Chornolysta by 26 %; the amount of roots increased in Lydia by 27 %, in Mama by 20 %, in Chornolysta by 31 %. The proposed modification of the nutrient medium МС for rhizogenesis which contains IAC and IBA at 0.5 mg/l enriched with silicon nanoparticles at a concentration of 5 mg/l has increased rhizogenesis frequency in Mentha piperita of Lydia and Mama varieties up to 100 %. Plants implantation degree is 100 %. Silicon nanoparticles concentration over 5 mg/l in the nutrient medium suppresses root formation under in vitro conditions, reduces plant rhizogenesis and implantation indices on the substratum.
Authors and Affiliations
Т. Є. Таланкова-Середа
Keywords
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