The Inhibitory Effect of Artemisa quttensis Extract on norA Efflux Pump in Ciprofloxacin Resistant Staphylococcus aureus Strains Using Ethidium Bromide and Real-Time PCR Methods
Journal Title: مجله علمی- پژوهشی دانشگاه علوم پزشکی قم - Year 2017, Vol 11, Issue 10
Abstract
Background and Objectives: norA efflux pump is one of the mechanisms of ciprofloxacin resistance in Staphylococcus aureus strains, and finding natural herbal compounds with the ability to inhibit efflux pumps is one of the recent challenges. The aim of this study was to determine the inhibitory effect of Artemisa quttensis extract on norA efflux pump gene expression in ciprofloxacin resistant S. aureus strains. Methods: At first, A. quttensis ethanolic extract, was prepared by ethanol solvent. Then, existence of norA efflux pump was detected in 10 ciprofloxacin resistant clinical strains of S. aureus using polymerase chain reaction (PCR) method. Moreover, anti-efflux activity of the extract was determined using ethidium bromide method. Finally, after treatment of isolates with Sub-MIC concentration of the extract, norA gene expression level, was evaluated using real-time PCR method. Results: The PCR results showed that all of the strains had norA efflux pump, and and ethidium bromide method revealed that the A. quttensis extract had inhibitory effect on resistant strains that had norA efflux pump. Moreover, real-time PCR results showed that norA gene expression level was down-regulated by Sub-MIC concentrations of the extract in the ciprofloxacin resistant isolates. Conclusion: Considering the anti-efflux pump effect of A. quttensis extract, it seems that this extract has the potential to be used as a native plant in pharmaceutical industries.
Authors and Affiliations
Amir Amir, Ali Asghar Ali Asghar, Hassan Hassan, Seyed Ataollah Seyed Ataollah
A Case Report of Tinea Capitis (Endothrix Type) in A 3 Year Old Boy Referred to Laboratory of Ali Ebne Abitaleb Hospital in Qom City (Iran)
Background and Objectives: Endothrix type of tinea capitis is one of the variety of dermatophytic infections of hair and scalp, which is more prevalent in boys, especially the age group of 6-11 years. The important point...
Correlation of PCSK9 and Serum sdLDL Levels and Other Demographic and Laboratory Indicators in Healthy Individuals
Background and Objectives: Atherosclerosis is a type of cardiovascular disease (CVD), which is known as the most important cause of death in the world. In atherosclerosis, arteries get thicker due to the entry of lipids...
Antibacterial activity of essential oil and extracts of Achillea tenuifolia against pathogenic bacteria
Background and Objectives: Chemical drugs have many side-effects, and for this reason, the approach of using medicinal plants and traditional medicine, has increased. Achillea tenuifolia is a plant belonging to the famil...
تاثیر کلاسهای آمادگی زایمان بر آگاهی و نگرش نسبت به نوع زایمان در زنان باردار شهر قم
چکیده زمینه و هدف: با توجه به اینکه در بسیاری از موارد عقاید و نگرش های نادرست، عدم آگاهی و ترس از زایمان طبیعی نوع زایمان را تعیین می کند، این پژوهش با هدف ارزشیابی تأثیر کلاسهای آمادگی زایمان بر آگاهی و نگرش نسبت به نو...
بررسی مقاومت آنتیبیوتیکی باکتریهای جداشده از محتویات و پوسته تخممرغهای صنعتی در شهر قم
زمینه و هدف: تخممرغ بهصورت مستقیم در سلامت تغذیهای خانواده تأثیر میگذارد. مهمترین آلودگیهای این محصول، حضور باکتریهای پاتوژن میباشد. این تحقیق با هدف ارزیابی میزان مقاومت آنتیبیوتیکی باکتریهای سالمونلا، اشرشیاک...