THE INHIBITORY EFFECT OF METHANOLIC AND AQUEOUS EXTRACTS OF TERMINALIA CHEBULA DRIED FRUITS ON METALLOBETALACTAMASES OF ACINETOBACTER BAUMANNII
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2014, Vol 6, Issue 9
Abstract
Objective: toevaluate Metallo-betalactamase (MBL) inhibitory effect and antimicrobial activity against Acinetobacter baumannii of methanolic and aqueous extracts of Terminaliachebuladried fruits.Methods: effect of EDTA (Ethylenediaminetetraacetic acid), methanolic and aqueous extracts of dried fruits of T. chebula on Metallo-betalactamase activity was studied in vitroby using cephaloridin asasubstrate. The degree of inhibition was determined byIC50and the antimicrobial activities of both extracts against Acinetobacter baumannii were qualitatively and quantitatively assessed by the presence or absence of inhibition zones, MIC and MBC values.Results: EDTA, methanolic and aqueous extracts inhibit metallo-betalactamases activity withIC50(3.98 mM, 18.45 and 19.68µg/ml), respectively. Polyphenols in aqueousand methanolic extracts are 513.094±3.061 and 496±2.856 µg GAE/mg, respectively.Conclusion: These results indicate that both extracts of T. chebula are rich in polyphenols and have a strong inhibitory effect on metallo-betalactamases in a competitive manner compared to EDTA. In addition, both extracts of T. chebula were also screened for their remarkable antibacterial and bactericidal activity against Acinetobacter baumannii confirmed by low minimum inhibitory and bactericidal concentration (MIC and MBC).Â
Authors and Affiliations
Selma Houchi, Rachid Mahdadi, Zakia Messasma, Farida Sahli
Keywords
Related Articles
- EP ID EP579643
- DOI -
- Views 70
- Downloads 0
DEVELOPMENT OF SUSTAINED RELEASE NANOCAPSULES OF CATECHIN RICH EXTRACT FOR ENHANCED BIOAVAILABILITY
Objective: The aim of the study was to develop sustained release nanocapsules of catechin rich extract (CRE) using Eudragit L 100 as a polymer by emulsion solvent evaporation technique with a major focus on enhancing its...
V. NEGUNDO, L. CAMARA AND B. VARIEGATA PLANTS LEAF EXTRACT EXHIBIT CONSIDERABLE IN VITRO ANTIOXIDANT AND ANTICANCER ACTIVITIES
Objective: The study was planned to investigate antioxidant and anticancer activities with the preliminary phytochemical analysis of methanolic extracts of Vitex negundo (V. negundo), Lantana camara (L. camara) and Bauha...
OPTIMIZATION OF LIPASE PRODUCTION FROM DIFFERENT AGROINDUSTRIAL WASTES BY MARINE ACTINOMYCETES
Objective: To identify the production of extracellular lipase by solid state fermentation (SSF) using coconut cake and groundnut cake using thermophilic strain of marine actinomycetes.Methods: From the isolated strains o...
PREPARATION, CHARACTERIZATION AND ANTIOXIDANT ACTIVITY OF XANTHONE-LOADED MAKING (HODGSONIA HETEROCLITA) MICROEMULSIONS
Objective: The aim of this study was to incorporate xanthone into Making (Hodgsonia heteroclita) microemulsions and to evaluate the antioxidant activity of the formulations.Methods: Making oil was obtained from the seed...
EFFECT OF PRENATAL ADMINISTRATION OF THERAPEUTIC DOSE OF TOPIRAMATE ON PLACENTAE ALBINO RATS' FETUSES
Objective: Topiramate is an antiepileptic drug (AED) used for the treatment of partial seizure in adult and children epileptic patients. It passed through the placenta causing a birth defect. However, little literature f...