The potential of serum fetal DNA for early diagnosis of gestational trophoblastic disease
Journal Title: Turkish Journal of Obstetrics and Gynecology - Year 2019, Vol 16, Issue 4
Abstract
Objective: To study cell-free DNA (cfDNA) levels in patients with gestational trophoblastic disease (GTD) in order to test the hypothesis that cfDNA circulating in maternal plasma could provide early detection of GTD. Materials and Methods: This study included 32 patients with GTD (complete mole and partial mole) and 30 non-GTD patients in the first trimester of pregnancy with no other medical problems. cfDNA levels in maternal serum were measured using polymerase chain reaction analysis on Y-chromosome–specific sequences. Results: cfDNA was found as 327±367 pg on average in the control group and 600±535 pg in the GTD group. Within the GTD group, the partial mole group had an cfDNA average of 636±549 pg, and the complete mole group had an cfDNA average of 563±536 pg. Although there was a statistically significant difference between the GTD group and the control group in terms of cfDNA (p=0.02), there was no statistically significant difference between the complete mole group and the partial mole group (p=0.76). Conclusion: Non-parametric analysis of covariance in terms of cfDNA in GTD was performed, thereby increasing its power and revealing a significant difference compared with the control group. This indicates that maternal peripheral bloodstream cfDNA monitoring might be significant in the early diagnosis of GTD.
Authors and Affiliations
Muhammed Hanifi Bademkıran, Özcan Balat, Seyhun Sucu, Mehmet Obut, Hüseyin Çağlayan Özcan, Fatma Bahar Cebesoy
Keywords
Related Articles
- EP ID EP684633
- DOI 10.4274/tjod.galenos.2019.54815
- Views 139
- Downloads 0
Hemorrhagic corpus luteum: Clinical management update
Hemorrhagic corpus luteum (HCL) is an ovarian cyst formed after ovulation and caused by spontaneous bleeding into a corpus luteum (CL) cyst. When HCL rupture happens, a hemoperitoneum results. Clinical symptoms are mainl...
Palliative pelvic exenteration using iliofemoral bypass with synthetic grafts for advanced cervical carcinoma
Objective: Recurrent cervical cancer can cause severe morbidity. Despite the severe morbidity after surgery, pelvic exenteration is still used today for mainly curative intent. This intention is neither based on randomiz...
Systematic review and meta-analysis of ropivacaine use in laparoscopic hysterectomy
To assess the efficacy of all forms of ropivacaine administration for the management of pain and opioid use, specifically in patients undergoing laparoscopic hysterectomy. We searched PubMed, Cochrane CENTRAL, Web of Sci...
How to perform and interpret the lung ultrasound by the obstetricians in pregnant women during the SARS-CoV-2 pandemic
Objective: Evidence for the use of lung ultrasound scan (LUS) examinations in coronavirus 2019 pneumonia is rapidly growing. The safe and non-ionizing nature of LUS drew attention, particularly for pregnant women. This s...
Is iodized salt efficient to overcome iodine deficiency in pregnants?
Objective: Iodine is a trace element that synthesizes thyroid hormones necessary for optimal human growth and development. The relationship between dietary iodine intake and spot urinary iodine excretion in pregnant wome...