The problem of Pseudomonas aeruginosa antibiotic-resistance and search of effective means of fighting
Journal Title: Бюлетень "Ветеринарна біотехнологія" - Year 2018, Vol 32, Issue
Abstract
Introduction. Pseudomonas aeruginosa (P. aeruginosa) is one of the “traditional” pathogens of purulent-septic diseases and complications, which causes about 30–70% of nosocomial infection cases. The causative agent has many pathogenicity factors and a complex of mechanisms for the development of resistance to antibiotics. P. аeruginosa is a pathogen for humans, animals and plants. Pseudomonosis is an acute contagious infectious disease of agricultural animals, fur animals, pets, birds, foxes, arctic foxes and fish. P. aeruginosa causes an infectious disease of young animals in the form of septicemia, toxemia with high mortality, and embryos death mainly in the last days of incubation. In adult animals Pseudomonas infection is characterized by the development of septicemia, pneumonia, encephalitis, aerosaculate, conjunctivitis, necrotic dermatitis, arthritis, hepatitis. The disease causes significant economic losses in livestock and poultry. The goal of the work was to study antibiotic resistance of P. aeruginosa in humane and veterinary medicine and research the effectiveness of the proposed anti–bacterial preparation Saroflox towards to P. aeruginosa. Materials and methods. Sensitivity of P. aeruginosa isolates to antibacterial preparations was determined by diffusion method in agar. The effectiveness of the proposed experimental antibiotic “Saroflox” against the isolates of P. aeruginosa was determined by the method of serial dilutions in a liquid nutrient medium – meat-pumped broth in comparison with enrofloxacin. Results of research and discussion. The presence of P. aeruginosa in the smears from the objects of the hospital environment (inpatient hospital, maternity hospital) and poultry farms (hatchery, chicken breeding department) has been confirmed. The polyresistance of P. aeruginosa isolates to antibacterial preparations has been established. A tendency to decrease in the sensitivity of the pathogen against the preparation of the group of cephalosporins, fluoroquinolones, aminoglycosides has been confirmed. Conclusions and prospects for further research. The effectiveness of the proposed anti–bacterial preparation Saroflox against P. aeruginosa in the concentrations from 0.078 to 10% and its more pronounced activity compared to enrofloxacin has been established.
Authors and Affiliations
Tatyana Fotina, Yevgenia Vashchyk, Roman Shcherbyna
Keywords
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- EP ID EP407680
- DOI 10.31073/vet_biotech32(2)-70
- Views 128
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