The role of Bak-mediated dissipation of mitochondrial membrane potential in radiosensitivity of Bax-negative human prostate cancer cells
Journal Title: Journal of Medical and Scientific Research - Year 2015, Vol 3, Issue 1
Abstract
In the radiotherapy of localised cancers, susceptibility of tumour and normal cells to undergo radiation-induced programmed cell death is an important prognostic factor. Mitochondria-mediated cell death is often preceded by dissipation of mitochondrial membrane potential. This study investigates whether human prostate cancer cells would preferentially die via the mitochondria-mediated pathway after photon and neutron irradiation. Bax-negative human prostate cancer cells were irradiated with p(66)/Be neutrons or 60Co -rays, in the absence or presence of an inhibitor of Bak translocation to mitochondria. Dissipation of mitochondrial membrane potential was assessed using the DePsipherTM kit. Cell survival was assessed using the colony forming assay. The dose response for the dissipation of mitochondria membrane potential was clearly dependent on radiation type, and the presence of the Bak inhibitor during irradiation almost completely abolished this process. This was paralleled by a significant enhancement in radioresistance in the presence of the inhibitor. These data indicate that, in Bax-negative tumours, Bak availability may lead to significant increases in the radiosensitivity, and can result in an enhanced therapeutic benefit.
Authors and Affiliations
Akudugu J, Serafin A
Keywords
Related Articles
- EP ID EP604624
- DOI 10.17727/JMSR.2015/3-005
- Views 60
- Downloads 0
Sitting is equivalent to smoking: The perils of sedentary lifestyle
Abstract is not available for this article
Sickle cell disease in pregnancy
Sickle cell disease (SCD) is a group ofinheritedsingle gene autosomal recessive disorder caused by single gene, which affects haemoglobin structure. SCD has its origin in sub-Saharan Africa and Middle East, hence it is m...
Fluid fluid levels in bone tumors and tumoral lesions - Pictorial essay
Fluid fluid level is an important and a rare finding noted on CT and MRI images of tumors and tumoral lesions of bone. Although this finding is commonly seen in primary aneurysmal bone cysts but also noted in other benig...
Emergency endovascular management of a leaking saccular abdominal aortic aneurysm
Abdominal aortic aneurysms (AAA) are relatively common, potentially life threatening conditions that are often asymptomatic and incidentally detected on routine screening for other problems. Vague abdominal pain and back...
Adenocarcinoma arising in a small bowel duplication cyst
Background: Enteric duplication cysts are uncommon congenital anomalies containing a normal gastrointestinal mucosa and smooth muscle layer that can occur anywhere throughout the digestive tract. Duplication cysts have t...