THE ROLE OF L-ALANINE SUPPLEMENTATION IN THE REGULATION OF HEPATIC GLUCONEOGENESIS IN RATS FED EITHER KETOGENIC AND/OR BIOTIN DEFICIENT DIET
Journal Title: International Journal of Medical and Clinical Research - Year 2011, Vol 2, Issue 2
Abstract
The purpose of this study was to evaluate the regulatory role of L-alanine supplementation at high level on hepatic gluconeogenesis in rats fed on either ketogenic diet and/or biotin deficient diet. Seven groups each of eight adult male Albino rats (Sprague-Dawely) strain, mean weight varied between 105.2. to 112.7 g were fed on basal control diet (CD), biotin deficient diet (BDD), ketogenic diet (KD), and ketogenic-biotin deficient diet (KBDD). L-alanine (L-Ala) was supplemented to biotin deficient and/or ketogenic diets at a level dose 10% throughout the experimental period (35 days). Plasma and liver were analyzed for biochemical parameters. The results showed that the plasma levels of glucose, insulin, free fatty acids (FFA), total proteins and liver glycogen were decreased significantly (p<0.01) in rats fed on biotin deficient (BDD) and ketogenic diets (KD and KBDD). Plasma β-hydroxybutyrate, acetoacetate, lactate, pyruvate, total cholesterol and triacylglycerol levels were significantly increased. Supplementation of L-alanine significantly restored these values near to normal value after 35 days of the experiment. Deficiency of biotin and feeding ketogenic diets reduced the activities of glucose-6-phosphatase (Glu-6-Pase), pyruvate carboxylase (PC), and phosphoenolpyruvate carboxykinase (PEP-CK) when compared to control. Addition of L-alanine increased the activity of Glu-6-Pase when compared to BDD and KD groups. The activities of PEP-CK and PC tended to be decreased in all tested rats when compared to control. The present study concluded that, supplementation of biotin-free diet and ketogenic diet with L-alanine stimulates glucose production and regulates hepatic gluconeogenesis.
Authors and Affiliations
FARES K. KHALIFA
Keywords
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- EP ID EP170034
- DOI 10.9735/0976-5530.2.2.84-92
- Views 116
- Downloads 0
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