The role of vitamin E in reducing aluminum hydroxide effects on testes of albino rats: a histological and immunohistochemical study
Journal Title: International Research Journal of Applied and Basic Sciences - Year 2016, Vol 10, Issue 4
Abstract
Objective: The objective of this study were to examine the histological changes in the testis after the administration of aluminum hydroxide and the possible protective role of vitamin E. Materials and methods: Thirty adult male albino rats were divided into three equal groups: control group; Aluminum hydroxide treated group received oral aluminum hydroxide at a dose of 30 mg/kg b. wt/day; (protected group) received both aluminum hydroxide (at the same previous dose) and an intraperitoneal injection of vitamin E at 50 IU/kg b. wt. At the end of the experiment (8 weeks), all animals were sacrificed and their testes were excised. Paraffin sections were prepared and stained with H&E, Periodic acid Schiff and immunohistochemical staining for proliferating cell nuclear antigen (PCNA). Morphometric and statistical analyses were carried out. Results: After the administration of aluminum hydroxide, some seminiferous tubules had disturbed basement membrane. The spermatogenic cells decreased in number to few layers and hyaline materials appeared within some tubules. Decreased PAS reaction and PCNA immunostaining and a significant decrease in the tubular parameters were recorded. The concomitant administration of vitamin E with aluminum hydroxide showed noticeable alleviation in histopathological changes induced by aluminum hydroxide in the structures of testis, increased PAS reaction and PCNA immunostaining and a marked increase in tubular parameters. Conclusion, the present study showed that exposure to aluminum hydroxide resulted in marked degenerative effects on the rats' testis and using vitamin E cannot completely prevent these effects of aluminum hydroxide, but it decreased to some extent the degenerative changes observed in testicular tissues.
Authors and Affiliations
Amal A. Afeefy| Anatomy Department, Faculty of Medicine, Alazhar University, Egypt, Marwa Salah| Department of Zoology, Faculty of Science, Beni-Suef University, Egypt, Amina M. A. Tolba| Anatomy Department, Faculty of Medicine, Alazhar University, Egypt
Keywords
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