The search for the mdr1-1Δ mutation of the MDR1 gene in four canine breeds in Uruguay (preliminary study)
Journal Title: Revista MVZ Cordoba - Year 2015, Vol 20, Issue 1
Abstract
Objective. The objective of this study is to analyze the frequency of mdr1-1Δ mutation in German Shepherd, Doberman, Border Collie and Greyhound dog breeds in Uruguay. Materials and methods. A total of 95 animals from the four breeds mentioned above were studied. DNA was isolated from blood using potassium acetate with a subsequent degradation from RNA with RNAsaH. The concentration and quality of the DNA obtained was evaluated with a Nanodrop, ND-1000 spectrophotometer. To determine the presence or absence of the mdr1-1Δ mutation, DNA samples were sent to Gene Seek, Neogen Corporation of Chicago, United States, for genotyping. Results. In all 95 animals studied, the mdr1-1Δ mutation was not present. Conclusions. Based on the preliminary results obtained, other elements that may cause adverse drug reactions must be considered: unidentified mutations in other regions of the MDR1 gene; mutations in other genes involved in the transport of drugs from the same subfamily or another; mutations in enzymes involved in drug metabolism (e.g. Cytochrome P450). Moreover, especially with Border Collies and Greyhounds, it is advisable to increase the number of animals in the study.
Authors and Affiliations
Rosa Gagliardi B, M. Sc *| Universidad de la República, Facultad de Veterinaria, Área Genética. Montevideo, C.P. 11600, Uruguay.Correspondencia: rgagliar@gmail.com, Diana Martínez A, MV| Veterinarios con ejercicio liberal de la profesión., Beatriz Tellechea H, MV| Veterinarios con ejercicio liberal de la profesión., Pedro Sitjar Q, MV| Veterinarios con ejercicio liberal de la profesión., Silvia Llambí D, Ph. D| Universidad de la República, Facultad de Veterinaria, Área Genética. Montevideo, C.P. 11600, Uruguay., María Arruga L, Ph. D| Universidad de Zaragoza, Facultad de Veterinaria, Laboratorio de Citogenética y Genética Molecular. Zaragoza, C. P. 50013, España.
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