Effect of glucose concentration on sperm motility activation in bocachico Prochilodus magdalenae (Pisces, Characiformes)
Journal Title: Revista MVZ Cordoba - Year 2011, Vol 16, Issue 2
Abstract
Objective. To determine the concentration of non-activating glucose on sperm motility as a component of future extender for cryopreservation of Prochilodus magdalenae semen. Materials and methods. Inactive semen obtained by hormonal induction from 3 males was analyzed. From each male, 0.25 μL of semen was deposited on Makler’s chamber, and then a fixed amount of 75 μl of glucose solution was added to each drop given a final concentration of, 0, 1, 2, 3, 4, 5, 6, 7, 8, 9 and 10%. Each male was checked 3 times (n=3). Osmolarity was determined in each seminal sample (~250-300 mOsm/Kg) and into each solution, (0, 62, 124, 186, 250, 310, 360, 410, 472, 536 y 620 mOsm/kg, respectively. The software Sperm Class Analyzer (SCA) was used to determine the curvilinear (VCL μm/sec) and straight (VSL, μm/sec) velocity (VSL, μm/sec), likewise, the rate of sperm motility (rapid, medium, and slow) was determined. Rate of immotile sperm was also determined. The activation time of motility (seconds) was obtained under light-field microscope. Results. Glucose Concentrations of 0 and 5% caused activation of sperm motility. Sperm activation was not visually detected in samples with glucose concentrations of 6% or higher. However, the SCA detected a total sperm motility of 7.2%, a VCL of 5.1 μm/sec and a VSL of 1.7 μm/sec. The SCA detected 100% non motile sperm in samples with 7% glucose solution and the motility was not subsequently acquired when distilled water was added. Conclusions. Glucose concentration has a determining effect on viability and activation of fresh sperm. Concentration of 6% glucose solution can be use as a non-activating solution for sperm motility in extenders for sperm cryopreservation in this specie.
Authors and Affiliations
Gregorio Martínez, M. Sc| Universidad Nacional de Colombia, FCA/DPA, Grupo de Investigación en Biodiversidad y Genética Molecular - BIOGEM, Sede Medellín, Calle 59A No. 63-020, Medellín, Colombia., Víctor Atencio G, M. Sc| Universidad de Córdoba, FMVZ/DCA, Centro de Investigación Piscícola – CINPIC, Carrera 6 No. 76-103, Montería, Colombia., Sandra Pardo C, Ph. D *| Universidad Nacional de Colombia, FCA/DPA, Grupo de Investigación en Biodiversidad y Genética Molecular - BIOGEM, Sede Medellín, Calle 59A No. 63-020, Medellín, Colombia.Correspondencia: scpardoc@unal.edu.co.
Keywords
Related Articles
- EP ID EP3205
- DOI -
- Views 390
- Downloads 16
MEDICAL AND NUTRITIONAL TREATMENT IN A PATIENT WITH HEPATIC ENCEPHALOPATHY OF POSIBLE PORTOCAVAL ORIGEN.
This article describes a case of a 3 month old female miniature schnauzer that exhibited excessive salivation, ataxia and disorientation after consuming puppy food. The physical examination revealed delayed growth for th...
Coat genetic markers of the domestic cat Felis catus (Felidae) from southwestern Colombia
Objectives. Establish the genetic profiles of cats from 12 neighboring municipalities in southwestern Colombia, in a town course from Pereira-Popayán. Estimate the degree of diversity, genetic structure, and quantify gen...
AISLAMIENTO DE Rickettsia bellii A PARTIR DE GARRAPATAS Amblyomma ovale Y Amblyomma incisum PROCEDENTES DEL SUR DE BRASIL
Objective. To isolate and characterize rickettsiae from the ticks Amblyomma ovale and Amblyomma incisum collected in the state of São Paulo. Materials and methods. Adult, free-living A. ovale and A. incisum were collecte...
Aflatoxicosis in chinchillas (Chinchilla lanigera)
Objetive. To determine the effects of dietary aflatoxins (AF) on chinchillas (Chinchilla lanigera). Materials and methods. A total of 20 male chinchillas were distributed in 5 experimental groups of 4 animals each. Anima...
SPERMATIC VARIABLES IN COLOMBIAN CREOLES BULLS COSTEÑO WITH HORNS AND ROMOSINUANO
In the Center of Investigation «Turipaná,» of the Colombian Corporation of Agricultural Investigation (CORPOICA), located to the north from Colombia; 8º31' north latitude and 75º49' west latitude. The variables spermatic...