The study of synergistic antileishmanial effect of Portulaca oleracea herb leaves and stems and Medicago lupulina leaves essence and alcoholic extract on a number of clinical strain of Leishmania major in vitro
Journal Title: International Journal of Farming and Allied Sciences - Year 2017, Vol 6, Issue 1
Abstract
Background and objective: Leishmaniasis has caused the world health problems with high endemicity in developing countries such as Iran. Various chemical drugs have been used for leishmaniasis treatment, but their side effects and drug resistance haveled to look for new effective compounds. Portulacaoleracea and Medicago lupulina, the traditional and medicinal herb- are two valuable source of new Pharmaceutical agents. Material and Methods: The essences were prepared through water distillation and alcoholic extracts were prepared through maceration method, then these were dried, and essences diluted with DMSO 5% and extracts solved in water and DMSO 5%. Leishmania major promastigotes were cultured in 2±25 °C temperature in the Schneider's medium, then in the stationary phase of RPMI-1640 culture growth medium, enriched with 10% fetal calf serum and Penicillin-Streptomycin to provide large quantity of them. Then the biological activity of composed of essences and extracts were evaluated on L.major promastigotes compared to glucantime drug using MTT colorometry. The optical density was measured with Eliza reader set, and the IC50 value was calculated. All tests repeated 3 times. Resalts and Discussion: Glucantime IC50 was 9 μg/ml after 48 hours respectively. After 48 hours, IC50 for composed of essences and extracts was 80 μg/ml against Leishmania promastigotes respectively. Although the glucantime pharmaceutical drug was more efficient compared to investigate composed of essences and extracts, the composed of them also had significantly effects on leishmania major promastigotes with higher density. Conclusion: Regarding that the studied composed of essences and extracts had considerable antileishmanial effect compared to glucantime in vitro, the necessity of conducting more experiments to investigate its effect on the parasite in animal model is also appreciated.
Authors and Affiliations
Elham Gharirvand Eskandari, Monir Doudi, Parisa Shoaei, Shervin Ghaffari, Majid Yaran
Keywords
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