The Use of Bacillus Amyloliquefaciens to Control of Sclerotinia Stem Rot (Sclerotinia Sclerotiorum) of Cucumber
Journal Title: International Journal of Farming and Allied Sciences - Year 2013, Vol 2, Issue 22
Abstract
Sclerotinia sclerotiorum (Lib.) de Bary is a soil-borne pathogen capable of infecting more than 400 host plants worldwide. Antagonistic rhizobacteria, more specifically certain species of Bacillus, are known to control of fungal root diseases of agronomic crops (Ashwini and Srividya, 2013) . In this study, 182 bacteria were isolated of cucumber rhizosphere and screened as potential biological control agents against S. sclerotiorum in vitro condition. Morphology, physiological and biochemical tests and 16S rDNA analysis demonstrated that Bacillus amyloliquefaciens significantly reduce the mycelial growth of S. sclerotiorum by 72.04% . Non-volatile compounds of 3 isolates showed noticeable inhibition zone (>36%) against S.sclerotiorum, whereas volatile compounds of 7 isolates could prevent more than 30% of the mycelial growth of the fungus. All strains except of FE1 promoted significantly plant growth in comparision to non-treated control. The population density on roots of plants treated with PE and AE reached to 8.40 and 7.81 log10 CFU g−1 root respectively. The use of bacteria as a soil treatment (PE 69.6%) was more effective to suppress the disease than those as a seed treatment (PE 62.3%). These results indicate that bacterial species used in this study could be used in the control of S. sclerotiorum.
Authors and Affiliations
Sharie Rostami, Mojdeh Maleki, Dariush Shahriari
Keywords
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