The validation of microscopic techniques for identification and differentiation of Microsporidia
Journal Title: European Journal of Medical Technologies - Year 2014, Vol 2, Issue 1
Abstract
Organisms belonging to the phylum Microsporidia in the regnum Fungi are causative agents of many serious diseases of human and animals. Most of the Microsporidia, which infect mammals can be the cause of human infestation, therefore for study this dangerous pathogens as a model organism was selected an insect – honey bee (Apis mellifera) and its two microsporidian parasites: Nosema apis and N. ceranae, which are the causative agents of nosemosis (a widespread contagious disease of bees). In this study observation of infected bees samples under bright-field, differential interference contrast (DIC), and phase contrast microscopy were compared. Furthermore, Nosema apis and N. ceranae spores were observed under Scanning Electron Microscopy (SEM), which allowed unequivocal identification of species. SEM of intestines samples revealed a formation of a layer made of microsporidian spores. Such a layer can be formed during other microsporidian infection located in intestines, as in the case of e.g. Encephalitozoon intestinalis, which is the cause of chronic diarrhoea and enteritis in AIDS patients and can be the cause of malnutrition and higher mortality among AIDS patients with gastrointestinal microsporidiosis.
Authors and Affiliations
Aneta A. Ptaszyńska
Keywords
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