The viability and intestinal epithelial cell adhesion of probiotic strain combination -[i] in vitro[/i] study
Journal Title: Annals of Agricultural and Environmental Medicine - Year 2012, Vol 19, Issue 1
Abstract
To be effective, probiotic bacteria must exhibit a number of functional characteristics, including the resistance to gastric acidity and the ability to adhere to the intestinal epithelium. In this study, we examined [i]in vitro[/i] the viability of lactic acid bacteria (LAB) combination after exposure to low pH, and the adhesion of LAB to Caco-2 cells during coincubation of 9 bacterial strains. To test bacterial viability, 6 commercially available products were incubated in 0.1 N HCl at pH 1.2 for 60 min. The greatest growth inhibition was noted for the non-capsulated product containing the Lactobacillus rhamnosus strain (log reduction of CFU = 6.4), and the best survival observed for the product containing 9 bacterial strains, equipped with a modern capsule made according to the Multi-Resistant Encapsulation technology (log reduction of CFU = 0.1). In the adhesion experiment, the combination of 9 bacterial strains was added to 17-day-old Caco-2 cell culture for 90 min. The greatest efficiency of adhesion was observed for the inoculum containing 5.5x10[sup]8[/sup] CFU/mL/9.6 cm[sup]2[/sup] of Caco-2 and the dose of probiotic bacteria of 190 cells per one Caco-2 cell. As a result, approximately 157 bacterial cells adhered to one Caco-2 cell. The results indicate that the combination of 9 bacterial strains in the examined product is characterized as highly adhesive.
Authors and Affiliations
Jacek Piątek, Magdalena Gibas-Dorna, Anna Olejnik, Hanna Krauss, Krzysztof Wierzbicki, Wioletta Żukiewicz-Sobczak, Maciej Głowacki
Keywords
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