The ways to reduce contamination of microscopic fungi in bee pollen
Journal Title: Біологія тварин - Year 2018, Vol 20, Issue 1
Abstract
The article describes different ways of reducing the contamination of microscopic fungi in bee pollen, that had qualitative physicochemical indices regarding water content, flavonoids content, active acidity and the content of crude protein according to the national standard 3127-95, but with 2.5–10 times high content of microscopic fungi. Mycological research was carried out on Sabouraud agar. The method of mycological research of experimental (treated) and native (raw) samples consisted in the fact that the collected 1 g of bee pollen was placed in a flask, where 0.9 % NaCl solution was added and stirred for 10–20 min. Than we aseptically transferred 1 cm3 of suspension in Petri dishes, poured molten and cooled agar, carefully mixed the suspension with the nutrient medium in circular motions. At the same time, a study of 6 samples of bee pollen was conducted using freezing, infrared irradiation and honey dilution for recording microscopic fungi. Grinding on an electric mill, freezing and defrosting 3 times of bee pollen do not allow reducing substantially the content of microscopic fungi in the forehead. Dilution of 10 times by honey in bee pollen significantly (P<0.01) reduces the content of fungi by 7.9 times, and 20 times dilution with honey causes a significant (P<0.01) decrease in microscopic fungi by 13.94 times. In case of contamination of the bee pollen with microscopic fungi by the genera Mucor and Rhizopus and the absence of contamination by Aspergillus genera, its safety can be increased by exposing infrared rays for 1 hour or by making 10 % honey-pollen product for use by humans or animals. It is necessary to conduct mycological control of bee pollen, if using bee pollen for the manufacture of candy for bees and honey-pollen products for people. If, however, the bee pollen is contaminated by fungi of the genus Aspergillus, it should be subjected to an additional study on the possibility of toxin formation.
Authors and Affiliations
O. Zastulka
Keywords
Related Articles
- EP ID EP356818
- DOI 10.15407/animbiol20.01.049
- Views 75
- Downloads 0
Effect of different compounds of trace elements on lipid composition of weaned piglets plasma
The article presents the study of lipid metabolism in comparing the actions of citrate salts, trace elements and micronutrients inorganic acids in the blood plasma of pigs lets. The study was conducted in 3 groups of pi...
Peroxidation processes in the blood, liver and skeletal muscles of rabbits with acute L-arginine-induced pancreatitis and its correction
Aim of this study was to investigate the processes of peroxidation in the blood, liver and skeletal muscles of rabbits with acute L-Arginine-induced pancreatitis and its correction by feeding linseen oil. The experiment...
The content of fatty acids in the bodies of Cyprinus carpio L. in different conditions of life
An important role in the processes of adaptation of living organisms to extreme conditions of the environment belongs to fatty acids (FA). By the method of gas chromatography with a flame-ionization detector we have exam...
The dynamics of the activity of chorionic gonadotropin stabilized by mannitol and sucrose at prolonged conservation
The studies of effects of various doses of mannitol and sucrose on the preservation of the activity of dissolved human chorionic hormone (HCH) have been performed. Human chorionic hormone from urine of pregnant women (12...
Sex steroids of blood in cows with purulonecrotic lesions of lower limbs during the reproductive cycle
The research objects were productive cows of Black Spotted Ukrainian Milk and Holstein breeds. Hormones in the blood of healthy cows were determined with immunofermental analysis during pregnancy (3rd, 5th and 9th months...