Time-Dependent Bactericidal Efficacy of Hydrogen Peroxide against Methicillin-Resistant Staphylococcus Aureus and Acinetobacter Baumannii in Second Degree Burn Wound
Journal Title: Biomedical Journal of Scientific & Technical Research (BJSTR) - Year 2019, Vol 15, Issue 4
Abstract
Nosocomial infections are major health care problem and their developing antibiotics resistance have made it harder to treat. Hydrogen peroxide (H2O2) is widely used for contaminated wound sterilization, but its efficacy and time of sterilization against Methicillin-Resistant Staphylococcus aureus (MRSA) and Acinetobacter baumannii (A. baumannii) in burn wound has yet to be fully determined. In this study, we investigated the time-dependent bactericidal activity of 3% hydrogen peroxide. In vitro studies, MRSA and A. baumanniitreated with hydrogen peroxide for 30 sec led to 100% bacterial kill. For in vivo studies, second degree burns was created on the back of mice followed by inoculation with 1 x 108 CFU ml-1 of MRSA and A. baumannii, isolated from clinical specimens of burn patients. Hydrogen peroxide application efficiently reduced bacterial burden 6.9 x 105 CFU/g (control: 7.5 x 1010 CFU/g) for 30 sec to 3.2 x 104 CFU/g (control: 9.2 x 109 CFU/g) for 120 sec of application duration. The log10 CFU bacterial load reduction with hydrogen peroxide application from 30 sec to 120 sec was found to be 0.96 (P<0.001). Furthermore, histological evaluation of the hydrogen peroxide (3%) treated and untreated burn wounds did not exhibit any significant tissue alterations. These results emphasize the potential use of 3% hydrogen peroxide as a possible treatment option for the decolonization of MRSA and A. baumannii bacteria in burn wounds without harming the tissue viability.
Authors and Affiliations
Abidullah Khan, Guohui Xiao, Amin Khan, Chunmao Han
Keywords
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- EP ID EP587649
- DOI 10.26717/BJSTR.2019.15.002720
- Views 185
- Downloads 0
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