Time-kill kinetics and biocidal effect of Euclea crispa leaf extracts against microbial membrane
Journal Title: Asian Pacific Journal of Tropical Medicine - Year 2017, Vol 10, Issue 4
Abstract
Objective: To evaluate antimicrobial potential of the fractions partitioned from Euclea crispa leaf extract and determination of their impact on cell membrane disruption. Methods: Antimicrobial potentials were evaluated via susceptibility test, determination of minimum inhibitory concentrations (MICs) and time-kill kinetics of the potent fractions. Degree of membrane disruption was determined by the amount of proteins and nucleotides released from within the cells and SEM images of the membrane after 120 min of treatment. Results: The largest inhibition zone (25.5 ± 0.50 mm) was obtained by ethylacetate fraction against Aeromonas hydrophilla at 10 mg/mL. The lowest MIC (0.16 mg/mL) was exhibited by n-butanol and ethylacetate fractions against test bacteria while all fractions exhibited MIC values between 0.31 and 1.25 mg/mL against susceptible yeast. n-Butanol fraction achieved absolute mortality against Bacillus pumulis (B. pumulis) and Klebsiella pneumoniae (K. pneumoniae) after 90 and 120 min contact time respectively at 1 × MIC. Total mortality also achieved by n-hexane fraction against B. pumulis and K. pneumoniae after 90 and 120 min respectively at 2 × MIC. Ethylacetate fraction achieved absolute mortality against both bacteria after 120 min at 2 × MIC. n-Hexane fraction achieved total mortality against Candida albicans after 120 min at 1 × MIC. Maximum amount of proteins (0.566 mg/mL) was released from K. pneumoniae by n-butanol fraction at 2 × MIC after 120 min of treatment while the maximum amount of nucleotides released (4.575 mg) was from B. pumulis by n-hexane fraction under similar condition. Conclusion: This study suggests the leaf of Euclea crispa a source of bioactive compound with membrane attack as one of the mechanisms of its biocidal action.
Authors and Affiliations
Kazeem Adekunle Alayande
Keywords
Related Articles
- EP ID EP267406
- DOI 10.1016/j.apjtm.2017.03.022
- Views 87
- Downloads 0
Endogamous marriage and the prevalence of hemoglobin E in ethnic groups of northern Thailand
Objective: To investigate the impact of the endogamous marriage culture on the prevalence of the hemoglobin E (HbE) recessive variant. Methods: The prevalence of the hemoglobin E (HbE) recessive variant was determined by...
Antiviral activity of five Asian medicinal plant crude extracts against highly pathogenic H5N1 avian influenza virus
Objective To study the antiviral properties of the five Asian medicinal plants against in vitro infection by the highly pathogenic avian influenza virus (H5N1). Methods Crude extracts of Andrographis paniculata, Curcu...
Preparedness and management of emergency evacuation in natural disasters in the showcase of earthquakes
Buildings that are used as residential areas by humans, mines they work in, vehicles they travel by or snowy mountains they go for sports can turn into deadly traps within seconds due to earthquakes, landslides, tsunamis...
Autophagy plays a protective role in advanced glycation end productsinduced apoptosis of chondrocytes via regulation of tumor necrosis factor-α, nuclear factor-κB and reactive oxygen species
Objective: To study the adverse effects of advanced glycation end products (AGEs) on chondrocytes and the role of autophagy in this process. Methods: Chondrocytes were harvested from the human articular cartilage tissues...
Chloride channel involved in the regulation of curcumin-induced apoptosis of human breast cancer cells
Objective: To investigate the role of ClC-3 chloride channel in the proliferation of breast cancer cell line Mcf-7 treated with curcumin and its specific mechanism. Methods: MTT assay was used to detect the effect of chl...